UMBC Biotechnology at Shady Grove

Permanent URI for this collectionhttp://hdl.handle.net/11603/33158

Browse

Recent Submissions

Now showing 1 - 5 of 5
  • Thumbnail Image
    Item
    Neuroprotective Effect of 7,8-dihydroxyflavone in a Mouse Model of HIV-Associated Neurocognitive Disorder (HAND)
    (HSPI, 2024-09-18) Makar, Tapas K; Bryant, Joseph; Shim, Bosung; Keledjian, Kaspar; Davis, Harry; Ghosh, Manik; Koirala, Ajay; Ghosh, Ishani; Makar, Shreya; Heredia, Alonso; Lane, Malcolm; Simard, J. Marc; Gallo, Robert C.; Gerzanich, Volodymyr; Merchenthaler, Istvan
    Treatment for HIV-associated neurocognitive disorders (HAND) remains elusive. 7,8-dihydroxy lavone (DHF), an analog of brain-derived neurotrophic factor (BDNF) and a high-af inity TrkB agonist, has been proposed as a viable therapeutic alternative to BDNF in crossing the Blood-Brain Barrier (BBB) and promoting growth, differentiation, maintenance, and survival of neurons. Here, we expand on our previous study investigating the therapeutic role of DHF on the cortical and hippocampal brain regions of the Tg26 mice, an animal model of HAND. We detected increased immunoreactivity for ion channels (SUR1, TRPM4) and the water channel aquaporin-4 (AQP4), suggesting an ionic and osmotic imbalance in the brains of Tg26 mice. Tg26 mice also exhibited loss of synaptic stability (SYN, SYP) and nicotinamide metabolism (NAMPT, SIRT1) that were associated with astrogliosis. Furthermore, Tg26 mice demonstrated increased iNOS and reduced HO-1/NRF2 expressions, implicating increased ER and oxidative stress. DHF treatment in Tg26 mice reversed these pathological changes. These data suggest crosstalk among TrkB, Akt, and related transcription factors (NF-κB, STAT3, and NRF2) as an underlying mechanism of Tg26-associated pathology in the brain. Finally, taken together with our prior study, these results further highlight a therapeutic role of DHF in promoting neuroprotection in HAND that may be applied in conjunction with current antiviral therapies.
  • No Thumbnail Available
    Item
    Developing a Career-Focused, Diverse Mentoring Program for Underrepresented STEM Undergraduates
    (The University of New Mexico, 2024-06-01) Wayman, Annica; Stolle-McAllister, Kathleen; LaCourse, William
    The racial and gender composition of the STEM workforce and holders of advanced degrees still has low representation from those traditionally underrepresented groups (URGs) in STEM. Having little representation of URGs in the STEM workforce, especially among academic faculty, perpetuates the issue of STEM students having few, if any, diverse STEM faculty to engage which has been shown to hinder retention in STEM. While involving STEM students from URGs in research mentoring experiences with non-minority faculty mentors addresses this issue to some extent, it also may limit a student’s exposure to STEM careers beyond academic research which may help retain them. Thus, the UMBC STEM BUILD program developed and implemented a career-focused mentoring program that included a broad array of STEM professions from URGs to support career development of 2nd year STEM students.
  • Thumbnail Image
    Item
    A-VAX: Applying Quality by Design to Vaccines
    (CASSS, 2012-05) CMC Vaccines Working Group Consortium; Schenerman, Mark
  • Thumbnail Image
    Item
    A–Mab: A Case Study in Bioprocess Development
    (ISPE, 2009-10-30) Phillips, Joseph; Ericson, Lisa; Karunatilake, Chulani; Schenerman, Mark; et al
    This is a detailed case study to stimulate discussion around how the core principles contained in Q8(R2), Q9 and Q10 guidelines could be applied to product realisation programs for a biotechnology-derived monoclonal antibody.
  • No Thumbnail Available
    Item
    The Roles of Bioactivity Assays in Lot Release and Stability Testing
    (BioProcess International, 2010-06) Rieder, Noel; Gazzano-Santoro, Hélène; Schenerman, Mark; Strause, Robert; Fuchs, Chana; Mire-Sluis, Anthony; McLeod, Lorna D.