Proteome Analysis to Assess Physiological Changes in Escherichia coli Grown Under Glucose-Limited Fed-Batch Conditions

Babu Raman, M.P. Nandakumar, Vignesh Muthuvijayan, Mark R. Marten, Proteome Analysis to Assess Physiological Changes in Escherichia coli Grown Under Glucose-Limited Fed-Batch Conditions, BIOTECHNOLOGY AND BIOENGINEERING, VOL. 92, NO. 3, NOVEMBER 5, 2005, DOI: 10.1002/bit.20570

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"This is the peer reviewed version of the following article: Babu Raman, M.P. Nandakumar, Vignesh Muthuvijayan, Mark R. Marten, Proteome Analysis to Assess Physiological Changes in Escherichia coli Grown Under Glucose-Limited Fed-Batch Conditions, BIOTECHNOLOGY AND BIOENGINEERING, VOL. 92, NO. 3, NOVEMBER 5, 2005, DOI: 10.1002/bit.20570, which has been published in final form at https://onlinelibrary.wiley.com/doi/epdf/10.1002/bit.20570. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."

Subjects

fed-batch fermentation
proteome analysis
nutrient limitation
starvation
stationary phase
Escher-ichia coli

Abstract

Proteome analysis was used to compare global protein expression changes in Escherichia coli fermentation between exponential and glucose-limited fed-batch phase. Two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry were used to separate and identify 49 proteins showing>2-fold difference in expression. Proteins up regulated during exponential phase include ribonucleotide biosynthesis enzymes and ribosomal recycling factor. Proteins up regulated during fed-batch phase include those involved in high-affinity glucose uptake, transport and degradation of alternate carbon sources and TCA cycle, suggesting an enhanced role of the cycle under glucose- and energy-limited conditions. We report the up regulation of several putative proteins (ytfQ,ygiS,ynaF,yggX,yfeX), not identified in any previous study under carbon-limited conditions.