Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3457482/

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https://doi.org/10.1128/AEM.01794-12
 http://hdl.handle.net/11603/13127

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UMBC Department of Marine Biotechnology
UMBC Biological Sciences Department
UMBC Faculty Collection

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Date

2012-10

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journal articles
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Citation of Original Publication

Harold J. Schreier, Wipawee Dejtisakdi, Jose Ortega Escalante, and Marina Brailo, Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant, Appl Environ Microbiol. 2012 Oct; 78(19): 7120–7123, DOI: 10.1128/AEM.01794-12

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Subjects

Planctomyces limnophilus
EZ-Tn5 transposome
phosphoenolpyruvate carboxykinase

Abstract

A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluconeogenic role for pckA in P. limnophilus.