Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant
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2012-10
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Harold J. Schreier, Wipawee Dejtisakdi, Jose Ortega Escalante, and Marina Brailo, Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant, Appl Environ Microbiol. 2012 Oct; 78(19): 7120–7123, DOI: 10.1128/AEM.01794-12
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Abstract
A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluconeogenic role for pckA in P. limnophilus.