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dc.contributor.advisorHsieh, Wang-Ting
dc.contributor.advisorBoyd, Ann
dc.contributor.advisorLaufer, Craig
dc.contributor.authorPoore, Allison
dc.contributor.departmentBiomedial Scienceen_US
dc.contributor.programHood College Graduate School Biomedical Science Programen_US
dc.date.accessioned2019-03-22T17:37:40Z
dc.date.available2019-03-22T17:37:40Z
dc.date.issued2019-03-22
dc.description.abstractMouse fur mites Myobia musculi and Myocoptes musculinus are common parasites of mice and present a unique challenge for exclusion from laboratory animal facilities. In this study, a real-time PCR test was developed to detect both mouse fur mites using distinct dye in a single reaction well. Confirmatory endpoint PCR tests were also designed, to confirm equivocal results. The assays were then tested during a pilot study, where known positive animals were used to evaluate the accuracy of environmental targets for screening. In all cages were mice were positive for the fur mite real-time PCR, environmental targets were also positive within 10 days of placement in a new, clean cage. This study shows that the real-time PCR assay and environmental targets are an effective combination for screening of mouse fur mites in laboratory animal facilities.en_US
dc.format.extent67 pagesen_US
dc.genreThesisen_US
dc.identifierdoi:10.13016/m2jehr-byvh
dc.identifier.urihttp://hdl.handle.net/11603/13129
dc.language.isoen_USen_US
dc.relation.isAvailableAtHood College
dc.subjectBiology, Molecular (0307)en_US
dc.titleDEVELOPMENT OF A REAL-TIME PCR ASSAY FOR MOUSE FUR MITES AND EVALUATION OF PILOT STUDYen_US
dc.typeTexten_US


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