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    Operon-specific regulation of ribosomal protein synthesis in Escherichia coli

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    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC411902/
    Permanent Link
    https://dx.doi.org/10.1073%2Fpnas.76.12.6542
    http://hdl.handle.net/11603/14010
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    • UMBC Biological Sciences Department
    • UMBC Faculty Collection
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    Author/Creator
    Lindahl, L.
    Zengel, J. M.
    Date
    1979-12
    Type of Work
    5 pages
    Text
    journal articles
    Citation of Original Publication
    L. Lindahl and J. M. Zengel, Operon-specific regulation of ribosomal protein synthesis in Escherichia coli, Proc Natl Acad Sci U S A. 1979 Dec; 76(12): 6542–6546. doi: 10.1073/pnas.76.12.6542
    Rights
    This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
    Subjects
    ribosomal proteins
    isopropylthiogalactoside (IPTG)
    Escherichia coli
    Abstract
    We have cloned a DNA fragment harboring the genes for ribosomal proteins L2, L4, and L23 on a plasmid vector that contains a lac operator and promoter. The cloned ribosomal protein genes are now under the control of lacOP. Addition of a lac inducer to these cells results in a specific 5- to 10-fold increase in the synthesis of the proteins corresponding to the cloned genes. Within 10 min of this induction, the synthesis of ribosomal proteins S3, S19, L3, L16, L22, and L29 stops almost completely. The genes for all these proteins reside in the same chromosomal operon as L2, L4, and L23. We have seen no dramatic effect on the synthesis of any other ribosomal proteins. Thus, the induction of L2, L4, and L23 results in a specific and rapid decrease in the expression of all (or almost all) genes in their own transcription unit.


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    Albin O. Kuhn Library & Gallery
    University of Maryland, Baltimore County
    1000 Hilltop Circle
    Baltimore, MD 21250
    www.umbc.edu/scholarworks

    Contact information:
    Email: scholarworks-group@umbc.edu
    Phone: 410-455-3544


    If you wish to submit a copyright complaint or withdrawal request, please email mdsoar-help@umd.edu.