High-efficiency, temperature-sensitive suppression of amber mutations in Escherichia coli

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC217294/
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http://hdl.handle.net/11603/14011
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Author/Creator
Date
1981-01
Type of Work
7 pages
Text
journal articles
Citation of Original Publication
J M Zengel and L Lindahl, High-efficiency, temperature-sensitive suppression of amber mutations in Escherichia coli, J Bacteriol. 1981 Jan; 145(1): 459–465, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC217294/
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Subjects
amber mutations
Escherichia coli
temperature-sensitive
Abstract
We have constructed a high-copy-number plasmid carrying an allele of the supD gene (supD43,74). The plasmid conferred temperature-sensitive suppression of amber mutations. Strains carrying the plasmid exhibited 50 to 60% suppression at 30 degrees C but little or no suppression at 42 degrees C. After a temperature shift from 30 to 42 degrees C the efficiency of suppression decreased gradually over a 60- to 90-min period before reaching the 42 degrees C steady-state level of suppression.