High-efficiency, temperature-sensitive suppression of amber mutations in Escherichia coli
Links to Fileshttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC217294/
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Type of Work7 pages
Citation of Original PublicationJ M Zengel and L Lindahl, High-efficiency, temperature-sensitive suppression of amber mutations in Escherichia coli, J Bacteriol. 1981 Jan; 145(1): 459–465, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC217294/
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We have constructed a high-copy-number plasmid carrying an allele of the supD gene (supD43,74). The plasmid conferred temperature-sensitive suppression of amber mutations. Strains carrying the plasmid exhibited 50 to 60% suppression at 30 degrees C but little or no suppression at 42 degrees C. After a temperature shift from 30 to 42 degrees C the efficiency of suppression decreased gradually over a 60- to 90-min period before reaching the 42 degrees C steady-state level of suppression.