The nucleotide sequence of the Escherichia coli fus gene, coding for elongation factor G.

Janice M. Zengel Richard H. Archer Lasse Lindahl, The nucleotide sequence of the Escherichta coli fus gene, coding for elongation factor G, Nucleic Acids Research, Volume 12, Issue 4, 24 February 1984, Pages 2181–2192, https://doi.org/10.1093/nar/12.4.2181

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Subjects

Escherichia coli fus gene
elongation factors G and Tu
ribosomal proteins

Abstract

We have determined the nucleotide sequence of the Escherichia coli fus gene, which codes for elongation factor G. The protein product of the sequenced gene contains 703 amino acids, with a predicted molecular weight of 77,444. The fus gene shows the nonrandom pattern of codon usage typical of ribosomal proteins and other proteins synthesized at a high level. We have identified several potential promoter sequences within the gene. One of these sequences may correspond to the secondary promoter for expression of the downstream tufA gene (encoding elongation factor Tu) whose activity has been described previously (1,2). A comparison of the nucleotide and amino acid sequences of elongation factors G and Tu reveals a limited but significant homology between the two proteins within the 150 amino acid residues at their amino-terminal ends.