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dc.contributor.advisorLaufer, Craig S.
dc.contributor.advisorEsposito, Dominic
dc.contributor.advisorHirschhorn, Ricky R.
dc.contributor.authorSanchez Hernandez, Jose
dc.contributor.departmentHood College Biology Departmenten_US
dc.contributor.programHood College Biomedical Science Programen_US
dc.date.accessioned2019-08-01T19:11:54Z
dc.date.available2019-08-01T19:11:54Z
dc.date.issued2019-08-01
dc.description.abstractSecond generation bioethanol using lignocellulosic waste is a promising source of renewable energy. Cellulose, lignin, and other biopolymers make up lignocellulose. Saccharification of lignocellulose requires cellulases, which have a catalytic domain (CD) and a carbohydrate binding module (CBM) that binds the enzyme to substrates. Cellulase activity is known to decrease during saccharification when CBMs irreversibly bind lignin that the CD cannot hydrolyze for release and further reactions. While costly, cellulases must be supplemented after each cycle of saccharification. Here we demonstrate that following denaturation when heated to 5°C above the melting temperature (Tm), CBMs 11 and 44 (CAZy families) from Hungateiclostridium thermocellum (CtCBM11 and CtCBM44) can be released from a bound substrate. Once cooled to a temperature below the Tm, CtCBM11 and CtCBM44, Type B CBMs with a β-sandwich fold, spontaneously refold and regain binding function. Using temperature tunable CBMs could drastically reduce saccharification costs by improving enzyme recycling strategies.en_US
dc.description.sponsorshipUSDAen_US
dc.format.extent81 pagesen_US
dc.genreThesis (M.S.)en_US
dc.identifierdoi:10.13016/m2csar-ngm9
dc.identifier.urihttp://hdl.handle.net/11603/14411
dc.language.isoen_USen_US
dc.relation.isAvailableAtHood College
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectCarbohydrate binding moduleen_US
dc.subjectGlycoside hydrolaseen_US
dc.subjectAbsolute contact orderen_US
dc.subjectSaccharificationen_US
dc.subjectEnzyme recyclingen_US
dc.subjectLignocelluloseen_US
dc.subjectLigninen_US
dc.subjectCatalytic domainen_US
dc.subjectHungateiclostridium thermocellumen_US
dc.subjectMelting temperatureen_US
dc.subjectCarbohydrate-active enzymesen_US
dc.subjectTemperature tunableen_US
dc.titleTHERMAL DENATURATION AND REFOLDING OF CARBOHYDRATE BINDING MODULES TO IMPROVE ENZYME RECYCLING IN A LIGNOCELLULOSIC BIOREFINERYen_US
dc.typeTexten_US


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Attribution-NonCommercial-ShareAlike 3.0 United States
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 United States