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    Acetylcholine Increases Intracellular Ca2+ in Taste Cells Via Activation of Muscarinic Receptors

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    https://journals.physiology.org/doi/full/10.1152/jn.2002.87.6.2643
    Permanent Link
    https://doi.org/10.1152/jn.2002.87.6.2643
    http://hdl.handle.net/11603/21073
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    • UMBC Biological Sciences Department
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    Author/Creator
    Ogura, Tatsuya
    Date
    2002-06-01
    Type of Work
    7 pages
    Text
    journal articles
    Citation of Original Publication
    Tatsuya Ogura, Acetylcholine Increases Intracellular Ca2+ in Taste Cells Via Activation of Muscarinic Receptors, J Neurophysiol 87: 2643–2649, 2002; 10.1152/jn.00610.2001
    Rights
    This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
    Subjects
    taste cells
    muscarinic receptors
    neurotransmitters
    nerve fibers
    Abstract
    Previous studies suggest that acetylcholine (ACh) is a transmitter released from taste cells as well as a transmitter in cholinergic efferent neurons innervating taste buds. However, the physiological effects on taste cells have not been established. I examined effects of ACh on taste-receptor cells by monitoring [Ca2+]i. ACh increased [Ca2+]i in both rat and mudpuppy taste cells. Atropine blocked the ACh response, butd-tubocurarine did not. U73122, a phospholipase C inhibitor, and thapsigargin, a Ca2+-ATPase inhibitor that depletes intracellular Ca2+stores, blocked the ACh response. These results suggest that ACh binds to M1/M3/M5-like subtypes of muscarinic ACh receptors, causing an increase in inositol 1,4,5-trisphosphate and subsequent release of Ca2+ from the intracellular stores. A long incubation with ACh induced a transient response followed by a sustained phase of [Ca2+]i increase. In Ca2+-free solution, the sustained phases disappeared, suggesting that Ca2+ influx is involved in the sustained phase. Depletion of Ca2+ stores by thapsigargin alone induced Ca2+ influx. These findings suggest that Ca2+ store-operated channels may be present in taste cells and that they may participate in the sustained phase of [Ca2+]i increase. Immunocytochemical experiments indicated that the M1 subtype of muscarinic receptors is present in both rat and mudpuppy taste cells.


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    Albin O. Kuhn Library & Gallery
    University of Maryland, Baltimore County
    1000 Hilltop Circle
    Baltimore, MD 21250
    www.umbc.edu/scholarworks

    Contact information:
    Email: scholarworks-group@umbc.edu
    Phone: 410-455-3021


    If you wish to submit a copyright complaint or withdrawal request, please email mdsoar-help@umd.edu.