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dc.contributor.advisorBozue, Joel
dc.contributor.advisorErwin-Cohen, Rebecca
dc.contributor.advisorBachert, Beth
dc.contributor.authorRodriguez, Sabrina
dc.contributor.departmentHood College Biologyen_US
dc.contributor.programHood College Biomedical and Environmental Scienceen_US
dc.date.accessioned2021-04-15T18:28:41Z
dc.date.available2021-04-15T18:28:41Z
dc.description.abstractFrancisella tularensis causes tularemia and is a potential biothreat agent with no licensed vaccine. Peptidoglycan, which makes up the cell wall, is a structure unique to bacteria and ideal target for medical countermeasures. Therefore, I sought to characterize the putative peptidoglycan-modifying enzymes murein lytic transglycosylase (mltA), and DD-carboxypeptidases (dacB1 and dacB2) in the surrogate strains Francisella novicida and the Live Vaccine Strain (LVS), and assess gene expression. LVS maintained relatively stable expression of these genes independent of environment. F. novicida, however, exhibited significant changes. dacB1 and mltA are essential for survival in LVS and therefore could serve as potential targets for inhibitors. LVS, along with virulent Francisella strains, contains a truncated version of dacB2, and complementation efforts showed a slight decrease in fitness, indicating the presence of a pathoadaptive mutation, but this requires further investigation. Altogether, these experiments show peptidoglycan-modifying enzymes continue to be viable targets for therapeutics against tularemia.en_US
dc.genreThesisen_US
dc.identifierdoi:10.13016/m29ufy-6q48
dc.identifier.urihttp://hdl.handle.net/11603/21346
dc.relation.isAvailableAtHood College
dc.rightsPublic Domain Mark 1.0*
dc.rights.urihttp://creativecommons.org/publicdomain/mark/1.0/*
dc.subjectFrancisella tularensisen_US
dc.subjectLVSen_US
dc.subjectPeptidoglycanen_US
dc.subjectLytic transglycosylaseen_US
dc.subjectCarboxypeptidaseen_US
dc.titleCHARACTERIZING PEPTIDOGLYCAN PROTEINS IN FRANCISELLA SPECIES TO IDENTIFY POTENTIAL THERAPEUTIC TARGETSen_US
dc.typeCollectionen_US


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