Advances in zinc sensors for studying zinc release events from pancreatic cells
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2006-10-25
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Rosenzweig, Zeev; Rosenzweig, Nitsa; Crivat, Georgeta; Advances in zinc sensors for studying zinc release events from pancreatic cells; Proceedings Volume 6380, Smart Medical and Biomedical Sensor Technology IV; 638002 (2006); https://www.spiedigitallibrary.org/conference-proceedings-of-spie/6380/638002/Advances-in-zinc-sensors-for-studying-zinc-release-events-from/10.1117/12.690653.short?SSO=1
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©2006 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited
©2006 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited
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Abstract
This paper describes the fabrication and characterization of the analytical properties of fluorescence-based zinc ion sensing glass slides and antibody based zinc sensors and their application in monitoring zinc release from beta pancreatic cells. The zinc ion indicator ZnAF-2 {6-[N- [N', N'-bis (2-pyridinylmethyl)-2-aminoethyl] amino-3',6'-dihydroxy-spiro[isobenzofuran-1(3H),9'-[9H] xanthene]-3-one} was modified to include a sufficiently long linking aliphatic chain, with a terminal carboxyl functional group. The activated carboxyl-modified ZnAF-2 was conjugated to the amino silanized surface of glass slides and to free amino groups of the A2B5 antibody molecules. The sensors were used to monitor zinc ion release events from glucose-stimulated pancreatic cells.