Advances in zinc sensors for studying zinc release events from pancreatic cells

Rosenzweig, Zeev; Rosenzweig, Nitsa; Crivat, Georgeta

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Date

2006-10-25

Type of Work

9 pages
Text
conference papers and proceedings

Citation of Original Publication

Rosenzweig, Zeev; Rosenzweig, Nitsa; Crivat, Georgeta; Advances in zinc sensors for studying zinc release events from pancreatic cells; Proceedings Volume 6380, Smart Medical and Biomedical Sensor Technology IV; 638002 (2006); https://www.spiedigitallibrary.org/conference-proceedings-of-spie/6380/638002/Advances-in-zinc-sensors-for-studying-zinc-release-events-from/10.1117/12.690653.short?SSO=1

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Abstract

This paper describes the fabrication and characterization of the analytical properties of fluorescence-based zinc ion sensing glass slides and antibody based zinc sensors and their application in monitoring zinc release from beta pancreatic cells. The zinc ion indicator ZnAF-2 {6-[N- [N', N'-bis (2-pyridinylmethyl)-2-aminoethyl] amino-3',6'-dihydroxy-spiro[isobenzofuran-1(3H),9'-[9H] xanthene]-3-one} was modified to include a sufficiently long linking aliphatic chain, with a terminal carboxyl functional group. The activated carboxyl-modified ZnAF-2 was conjugated to the amino silanized surface of glass slides and to free amino groups of the A2B5 antibody molecules. The sensors were used to monitor zinc ion release events from glucose-stimulated pancreatic cells.