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    Current advances in molecular, biochemical, and computational modeling analysis of microalgal triacylglycerol biosynthesis

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    https://www.sciencedirect.com/science/article/pii/S0734975016300726
    Permanent Link
    https://doi.org/10.1016/j.biotechadv.2016.06.004
    http://hdl.handle.net/11603/25014
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    • UMBC Biological Sciences Department
    • UMBC Chemistry & Biochemistry Department
    • UMBC Department of Marine Biotechnology
    • UMBC Faculty Collection
    • UMBC Student Collection
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    Author/Creator
    Lenka, Sangram
    Carbonaro, Nicole
    Park, Rudolph
    Miller, Stephen M.
    Thorpe, Ian
    Li, Yantao
    Author/Creator ORCID
    https://orcid.org/0000-0002-0121-2430
    https://orcid.org/0000-0002-2987-329X
    https://orcid.org/0000-0001-9614-4444
    https://orcid.org/0000-0001-7545-1883
    Date
    2016-07-14
    Type of Work
    44 pages
    Text
    journal articles
    postprints
    Citation of Original Publication
    Lenka, Sangram K. et al. Current advances in molecular, biochemical, and computational modeling analysis of microalgal triacylglycerol biosynthesis. Biotechnology Advances 34 (September–October 2016). mp 5, pp 1046-1063. https://doi.org/10.1016/j.biotechadv.2016.06.004
    Rights
    This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
    Subjects
    Triacylglycerols (TAGs)
    cellular and molecular factors regulating TAG biosynthesis
    unicellular 36 microalgae
    tools for genetic manipulation of microalgae
    Abstract
    Triacylglycerols (TAGs) are highly reduced energy storage molecules ideal for biodiesel production. Microalgal TAG biosynthesis has been studied extensively in recent years, both at the molecular level and systems level through experimental studies and computational modeling. However, discussions of the strategies and products of the experimental and modeling approaches are rarely integrated and summarized together in a way that promotes collaboration among modelers and biologists in this field. In this review, we outline advances toward understanding the cellular and molecular factors regulating TAG biosynthesis in unicellular microalgae with an emphasis on recent studies on rate-limiting steps in fatty acid and TAG synthesis, while also highlighting new insights obtained from the integration of multi-omics datasets with mathematical models. Computational methodologies such as kinetic modeling, metabolic flux analysis, and new variants of flux balance analysis are explained in detail. We discuss how these methods have been used to simulate algae growth and lipid metabolism in response to changing culture conditions and how they have been used in conjunction with experimental validations. Since emerging evidence indicates that TAG synthesis in microalgae operates through coordinated crosstalk between multiple pathways in diverse subcellular destinations including the endoplasmic reticulum and plastids, we discuss new experimental studies and models that incorporate these findings for discovering key regulatory checkpoints. Finally, we describe tools for genetic manipulation of microalgae and their potential for future rational algal strain design. This comprehensive review explores the potential synergistic impact of pathway analysis, computational approaches, and molecular genetic manipulation strategies on improving TAG production in microalgae.


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    Albin O. Kuhn Library & Gallery
    University of Maryland, Baltimore County
    1000 Hilltop Circle
    Baltimore, MD 21250
    www.umbc.edu/scholarworks

    Contact information:
    Email: scholarworks-group@umbc.edu
    Phone: 410-455-3021


    If you wish to submit a copyright complaint or withdrawal request, please email mdsoar-help@umd.edu.