Characterization of an Endogenous Primate Virus: Extent of Nucleic Acid Homology with Various DNAs
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Date
1978-09
Type of Work
Department
Hood College Biology
Program
Hood College Biomedical Science
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Abstract
A [
3
H]cDNA viral probe has been prepared from the baboon endogenous
virus isolate designated M7 via the endogenous reverse transcriptase
reaction. The M7 [
3
H]cDNA viral probe reassociated extensively and with
high thermal stability to both its original viral genomic RNA template
and DNA of baboon origin. Hybridization of this M7cDNA probe with baboon
(Papio cynocephalus) cellular DNAs obtained from established cell lines
and frozen tissues detected approximately 40 to 95 copies of viral-related
DNA sequences per haploid genome. Approximately 10 to 20 times fewer
copies of viral-related sequences were detected in the DNAs extracted
from cell lines exogenously infected with a baboon endogenous virus than
were detected in DNAs of P. cynocephalus origin. No correlation was
found to exist between the level of active virus production by established
baboon cell lines and the number of viral-related sequences detected in
their cellular DNAs. In addition, less than one copy of viral-related
sequences per haploid genome was detected in cellular DNA of mouse,
normal human, and leukemic human origin. All hybridization reactions
were conducted under conditions of low stringency in order to detect
even distantly related sequences. The ratio of cellular DNA to M7cDNA
used in all experiments was sufficient to detect at least one copy of
viral-related sequences per haploid genome.