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    OVEREXPRESSION OF ADENYLATE CYCLASE ISOFORMS ALTERS CELL SIGNALING PATHWAYS IN NF1-NULL MALIGNANT PERIPHERAL NERVE SHEATH TUMORS

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    Hughes_hood_0917P_10003.pdf (1.762Mb)
    Permanent Link
    http://hdl.handle.net/11603/7829
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    • Hood College Biomedical and Environmental
    • Hood College Student Works
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    Author/Creator
    Hughes, JoAnna
    Date
    2017-11
    Type of Work
    48 pages
    Text
    theses
    Department
    Biology
    Program
    Biomedical and Environmental Biology
    Subjects
    Adenosine Receptors
    Adenylate Cyclase
    ADORA
    Neurofibromatosis Type 1
    NF1
    PKA
    Abstract
    The second messenger, cyclic-AMP (cAMP), is produced by ten adenylate cyclase (ADCY) isoforms. Previous studies indicate that ADCY isoforms may lead to either activation or inhibition of cAMP-dependent protein kinase (PKA), cAMP expression is increased in response to neurofibromin (NF1) mutations, and malignant peripheral nerve sheath tumors (MPNST) express more ADCY isoforms. ADCY expression and function are modulated by four adenosine receptors (ADORA), which have different effects on ADCY. We aimed to overexpress ADCY 3, 6, 7, and 9 in different cell lines to dissect the mechanism of how cAMP expression is altered after NF1 loss. Overexpression of ADCY 7 significantly affected PKA activity. Overexpression of ADCY 9 led to increased expression of ADORA 1, 2B, and 3 in MPNST cells. Overexpressed ADCY 3 and 7 increased ADORA 3 expression as well. These data indicate that changes in ADCY and ADORA expression in MPNSTs may account for changes in cAMP expression after loss of NF1.


    Beneficial-Hodson Library
    Hood College
    401 Rosemont Avenue
    Frederick, MD 21701
    www.hood.edu


    If you wish to submit a copyright complaint or withdrawal request, please email mdsoar-help@umd.edu.

     

     

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    Beneficial-Hodson Library
    Hood College
    401 Rosemont Avenue
    Frederick, MD 21701
    www.hood.edu


    If you wish to submit a copyright complaint or withdrawal request, please email mdsoar-help@umd.edu.