Induction of Endogenous Type C Virus by Defective Herpes Simplex Virus
| dc.contributor.author | Brewer, Pamela P. | |
| dc.contributor.department | Hood College Biology | |
| dc.contributor.program | Biomedical and Environmental Science | |
| dc.date.accessioned | 2024-09-10T15:08:19Z | |
| dc.date.available | 2024-09-10T15:08:19Z | |
| dc.date.issued | 1978-02 | |
| dc.description.abstract | Herpes simplex virus type 1 (HSV-1), rendered defective by ultraviolet light (UV) irradiation or photodynamic treatment, induced endogenous type C virus from A1-2 cells. Infection of A1-2 cells, a clonal line of sarcoma positive, helper negative (S+H-) cells derived from the Balb/c mouse, with defective HSV-1 resulted in the activation of endogenous xenotropic type C virus, as determined by infectious center assay on permissive normal rat kidney (NRK) indicator cells. The amount of xenotropic virus induced depended on UV dose to HSV-1 or visible light dose to HSV-1 treated with the photosensitizing dye, proflavine sulfate. Certain aspects of activation with UV- irradiated HSV-1 were investigated to further characterize this induction and compare it to other chemical means of endogenous type C virus induction. Cell population growth experiments showed that optimum virus induction occurred when cells were in log phase. Investigation of the effect of metabolic inhibitors on UV- irradiated HSV-1 induction demonstrated a decrease in induction by both hydroxyurea, a DNA synthesis inhibitor, and actinomycin D, an RNA synthesis inhibitor, with actinomycin D having a greater inhibitory effect. | |
| dc.format.extent | 72 pages | |
| dc.genre | Thesis (M.S.) | |
| dc.identifier | doi:10.13016/m2vmh7-11md | |
| dc.identifier.uri | http://hdl.handle.net/11603/36159 | |
| dc.language.iso | en | |
| dc.title | Induction of Endogenous Type C Virus by Defective Herpes Simplex Virus | |
| dc.type | Text |