Surface Modification of Nerve Guide Conduits with ECM Coatings and Investigating Their Impact on Schwann Cell Response
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Pinzon-Herrera, Luis, John Magness, Hector Apodaca-Reyes, Jesus Sanchez, and Jorge Almodovar. “Surface Modification of Nerve Guide Conduits with ECM Coatings and Investigating Their Impact on Schwann Cell Response.” Advanced Healthcare Materials, 2024: 2304103. https://doi.org/10.1002/adhm.202304103.
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This is the peer reviewed version of the following article: Pinzon-Herrera, Luis, John Magness, Hector Apodaca-Reyes, Jesus Sanchez, and Jorge Almodovar. “Surface Modification of Nerve Guide Conduits with ECM Coatings and Investigating Their Impact on Schwann Cell Response.” Advanced Healthcare Materials, 2024: 2304103. https://doi.org/10.1002/adhm.202304103., which has been published in final form at https://doi.org/10.1002/adhm.202304103. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
Access to this item will begin on 02-23-2025.
Access to this item will begin on 02-23-2025.
Abstract
In this study, layer-by-layer coatings composed of heparin and collagen are proposed as an extracellular mimetic environment on nerve guide conduits (NGC) to modulate the behavior of Schwann cells (hSCs). The authors evaluated the stability, degradation over time, and bioactivity of six bilayers of heparin/collagen layer-by-layer coatings, denoted as (HEP/COL)₆. The stability study reveals that (HEP/COL)₆ is stable after incubating the coatings in cell media for up to 21 days. The impact of (HEP/COL)₆ on hSCs viability, protein expression, and migration is evaluated. These assays show that hSCs cultured in (HEP/COL)₆ have enhanced protein expression and migration. This condition increases the expression of neurotrophic and immunomodulatory factors up to 1.5-fold compared to controls, and hSCs migrated 1.34 times faster than in the uncoated surfaces. Finally, (HEP/COL)₆ is also applied to a commercial collagen-based NGC, NeuraGen, and hSC viability and adhesion are studied after 6 days of culture. The morphology of NeuraGen is not altered by the presence of (HEP/COL)₆ and a nearly 170% increase of the cell viability is observed in the condition where NeuraGen is used with (HEP/COL)₆. Additionally, cell adhesion on the coated samples is successfully demonstrated. This work demonstrates the reparative enhancing potential of extracellular mimetic coatings.