Elucidation of a non-thermal mechanism for DNA/RNA fragmentation and protein degradation when using Lyse-It

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dc.contributor.authorSantaus, Tonya M.
dc.contributor.authorGreenberg, Ken
dc.contributor.authorSuri, Prabhdeep
dc.contributor.authorGeddes, Chris D.
dc.date.accessioned2020-01-31T17:59:35Z
dc.date.available2020-01-31T17:59:35Z
dc.date.issued2019-12-02
dc.description.abstractRapid sample preparation is one of the leading bottlenecks to low-cost and efficient sample component detection. To overcome this setback, a technology known as Lyse-It has been developed to rapidly (less than 60 seconds) lyse Gram-positive and–negative bacteria alike, while simultaneously fragmenting DNA/RNA and proteins into tunable sizes. This technology has been used with a variety of organisms, but the underlying mechanism behind how the technology actually works to fragment DNA/RNA and proteins has hitherto been studied. It is generally understood how temperature affects cellular lysing, but for DNA/RNA and protein degradation, the temperature and amount of energy introduced by microwave irradiation of the sample, cannot explain the degradation of the biomolecules to the extent that was being observed. Thus, an investigation into the microwave generation of reactive oxygen species, in particular singlet oxygen, hydroxyl radicals, and superoxide anion radicals, was undertaken. Herein, we probe one aspect, the generation of reactive oxygen species (ROS), which is thought to contribute to a non-thermal mechanism behind biomolecule fragmentation with the Lyse-It technology. By utilizing off/on (Photoinduced electron transfer) PET fluorescent-based probes highly specific for reactive oxygen species, it was found that as oxygen concentration in the sample and/or microwave irradiation power increases, more reactive oxygen species are generated and ultimately, more oxidation and biomolecule fragmentation occurs within the microwave cavity.en_US
dc.description.sponsorshipThis work is supported by the National Institutes of Health UMBC Graduate Training Chemistry Biology Interface Fellowship (T32GM066706-15) to TMS. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en_US
dc.description.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0225475en_US
dc.format.extent2 filesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2exxm-leop
dc.identifier.citationSantaus, Tonya M.; Greenberg, Ken; Suri, Prabhdeep; Geddes, Chris D.; Elucidation of a non-thermal mechanism for DNA/RNA fragmentation and protein degradation when using Lyse-It; PLoS ONE 14(12); https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0225475;en_US
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0225475
dc.identifier.urihttp://hdl.handle.net/11603/17202
dc.language.isoen_USen_US
dc.publisherPLOSen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemistry & Biochemistry Department Collection
dc.relation.ispartofUMBC Institute of Fluorescence (IoF)
dc.relation.ispartofUMBC Student Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsAttribution 4.0 International (CC BY 4.0)*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleElucidation of a non-thermal mechanism for DNA/RNA fragmentation and protein degradation when using Lyse-Iten_US
dc.typeTexten_US

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