Engineering whiting events in culture: A microalgae-driven calcium carbonate and biomass production process at high pH and alkalinity with the marine microalga Nannochloropsis oceanica IMET1
dc.contributor.author | Lee, Yi-Ying | |
dc.contributor.author | Jonas, Lauren | |
dc.contributor.author | Hill, Russell | |
dc.contributor.author | Place, Allen | |
dc.contributor.author | Silsbe, Greg | |
dc.contributor.author | Hunsicker, Scott | |
dc.contributor.author | North, Elizabeth | |
dc.contributor.author | Li, Yantao | |
dc.date.accessioned | 2024-03-06T18:52:25Z | |
dc.date.available | 2024-03-06T18:52:25Z | |
dc.date.issued | 2024-01-18 | |
dc.description.abstract | The impact of whiting events on global inorganic carbon cycle and climate change is debatable at the current ocean pH. This work engineered whiting events in a high pH, high alkalinity microalgal culture for carbon dioxide capture and storage. When growing the marine microalga Nannochloropsis oceanica IMET1 in photobioreactors, culture alkalinity more than doubled, increasing from 72.5 mg L⁻¹ to a maximum level of 159.6 mg L⁻¹. At the same time, culture pH increased from 7.9 to 10.1 with concomitant calcium carbonate production. X-ray diffraction analysis revealed the precipitated calcium carbonate was primarily monohydrocalcite. The lab culture was scaled up to a 340-L bioreactor, in which Nannochloropsis ash-free dry weight productivities ranged from 25.1 to 51.4 g m⁻² d⁻¹ and a maximum monohydrocalcite productivity of 133.4 g m² d⁻¹ was recorded. In this system, Nannochloropsis biomass contained about 23.9 % lipids and the eicosapentaenoic acid content was about 1.8 %. Together, these results suggest the microalgae-driven calcium carbonate and biomass production process efficiently captures and stores atmospheric carbon dioxide in the form of calcium carbonate while producing valuable bioproducts. Study of the bacterial communities associated with the Nannochloropsis culture identified four dominant species Maricaulis maris, Marinisubtilis pacificus, Gracilimonas sp., and an uncultured bacterium in the OD1 phylum with interesting features that warrant further investigation. | |
dc.description.sponsorship | We thank Dr. Peter Zavalij, X-ray Crystallographic Center at University of Maryland College Park for XRD analysis. We thank the funding from The Bailey Wildlife Foundation (BWF) as well as the entire BWF project team and DOE Office of Fossil Energy and Carbon Management (FE-0031914, FE-0032188) for their support. | |
dc.description.uri | https://www.sciencedirect.com/science/article/pii/S2212982024000040 | |
dc.format.extent | 12 pages | |
dc.genre | journal articles | |
dc.identifier | doi:10.13016/m2rq73-ious | |
dc.identifier.citation | Lee, Yi-Ying, Lauren Jonas, Russell Hill, Allen Place, Greg Silsbe, Scott Hunsicker, Elizabeth North, and Yantao Li. “Engineering Whiting Events in Culture: A Microalgae-Driven Calcium Carbonate and Biomass Production Process at High pH and Alkalinity with the Marine Microalga Nannochloropsis Oceanica IMET1.” Journal of CO2 Utilization 80 (February 1, 2024): 102669. https://doi.org/10.1016/j.jcou.2024.102669. | |
dc.identifier.uri | https://doi.org/10.1016/j.jcou.2024.102669 | |
dc.identifier.uri | http://hdl.handle.net/11603/31854 | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | |
dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
dc.relation.ispartof | UMBC Faculty Collection | |
dc.relation.ispartof | UMBC Chemistry & Biochemistry Department | |
dc.relation.ispartof | UMBC Department of Marine Biotechnology | |
dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | Carbon capture and storage | |
dc.subject | Microbiologically induced calcium carbonate precipitation (MICP) | |
dc.subject | Monohydrocalcite | |
dc.subject | Photobioreactor | |
dc.subject | Whiting | |
dc.title | Engineering whiting events in culture: A microalgae-driven calcium carbonate and biomass production process at high pH and alkalinity with the marine microalga Nannochloropsis oceanica IMET1 | |
dc.type | Text | |
dcterms.creator | https://orcid.org/0000-0001-7545-1883 |