THE IN VITRO HOST RANGE OF SELECTED PORCINE VIRUSES USING CYTOPATHIC EFFECT AND IMMUNOFLUORESCENCE ENDPOINTS
Links to Files
Permanent Link
Collections
Author/Creator
Author/Creator ORCID
Date
Department
Hood College Biology
Program
Biomedcial and Environmental Science
Citation of Original Publication
Rights
Subjects
Abstract
There is an increased interest in the use of porcine organs, tissues, and
cells for xenotransplantation and other biotechnological processes; hence, the
possible risk of cross-species transmission and contamination. A comparative
study was carried out to determine the susceptibility of seven cell types to three
porcine viruses; porcine parvovirus (PPV), porcine adenovirus (PAV), and
transmissible gastroenteritis virus (TGE). In this research six cell lines were
considered; Chinese hamster ovary cells (CHO-K1), African green monkey cells
(Vero), human cells (293 and MRC-5), mouse cells (NIH/3T3), and bovine
turbinate cells (BT). PT-1 cells were used as a permissive cell line (control).
Porcine adenovirus infected all cell lines studied with the exception of CHO-K1
cells, whereas, TGE only infected MRC-5. BT and 293 tested positive for PPV
with both end points but Vero cells showed positive results for IFA and not
cytopathogenicity. The amplification of the viruses showed that certain cell lines
which were found to display cytopathic effects or immunostaining on inculcation
with the porcine virus were not able to sustain the viral replication through
several serial passages and therefore is not permissive for these viruses.