THE IN VITRO HOST RANGE OF SELECTED PORCINE VIRUSES USING CYTOPATHIC EFFECT AND IMMUNOFLUORESCENCE ENDPOINTS

Abstract

There is an increased interest in the use of porcine organs, tissues, and cells for xenotransplantation and other biotechnological processes; hence, the possible risk of cross-species transmission and contamination. A comparative study was carried out to determine the susceptibility of seven cell types to three porcine viruses; porcine parvovirus (PPV), porcine adenovirus (PAV), and transmissible gastroenteritis virus (TGE). In this research six cell lines were considered; Chinese hamster ovary cells (CHO-K1), African green monkey cells (Vero), human cells (293 and MRC-5), mouse cells (NIH/3T3), and bovine turbinate cells (BT). PT-1 cells were used as a permissive cell line (control). Porcine adenovirus infected all cell lines studied with the exception of CHO-K1 cells, whereas, TGE only infected MRC-5. BT and 293 tested positive for PPV with both end points but Vero cells showed positive results for IFA and not cytopathogenicity. The amplification of the viruses showed that certain cell lines which were found to display cytopathic effects or immunostaining on inculcation with the porcine virus were not able to sustain the viral replication through several serial passages and therefore is not permissive for these viruses.