Determining the specificity and sensitivity of previously identified human, gull, ruminant, and poultry genetic markers

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dc.contributor.authorTague, Jessica
dc.contributor.departmentBiological Sciencesen_US
dc.date.accessioned2018-06-15T14:15:23Z
dc.date.available2018-06-15T14:15:23Z
dc.date.issued2017
dc.description.abstractFecal contamination of environmental waters is of major concern due to its potential to contaminate shellfish and recreational waters, posing a threat to human health. Microbial source tracking (MS1) is an important methodology for detecting the source of fecal contamination by probing water samples for genetic markers uniquely associated with bacteria from a specific host. The level of performance of genetic markers is measured by testing their specificity and sensitivity which can vary due to geographic and environmental conditions. In this project four genetic markers were tested for their specificity (n=l5, 17, or 18) and sensitivity by analyzing the products of PCR reactions containing DNA from known fecal sources and primers that target each specific marker. It was determined that the human primers, HF183, were 89.5% specific and I00% sensitive. Gull primers, Gul12, were 70.5% specific and 75% sensitive. Lastly, the poultry primers, LA35, were determined to be 100% specific and 50% sensitive. An analysis of ruminant primers, RumBac2, was inconclusive; further processing will be completed.en_US
dc.format.extent28 pagesen_US
dc.genrethesesen_US
dc.identifierdoi:10.13016/M2C53F441
dc.identifier.urihttp://hdl.handle.net/11603/10919
dc.language.isoen_USen_US
dc.relation.isAvailableAtSalisbury Universityen_US
dc.subjectEnvironmental waters -- Fecal contaminationen_US
dc.subjectMicrobial source trackingen_US
dc.titleDetermining the specificity and sensitivity of previously identified human, gull, ruminant, and poultry genetic markersen_US
dc.typeTexten_US

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