Exogenous modulation of intrinsic optic nerve neuroprotective activity

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https://link.springer.com/article/10.1007/s00417-010-1336-7

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https://doi.org/10.1007/s00417-010-1336-7
 http://hdl.handle.net/11603/39066

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UMBC Chemical, Biochemical & Environmental Engineering Department
UMBC College of Engineering and Information Technology Dean's Office

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https://orcid.org/0000-0002-0644-744X

Date

2010-08-01

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journal articles
postprints
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Citation of Original Publication

Grozdanic, Sinisa D., Tatjana Lazic, Markus H. Kuehn, Matthew M. Harper, Randy H. Kardon, Young H. Kwon, Erin B. Lavik, and Donald S. Sakaguchi. “Exogenous Modulation of Intrinsic Optic Nerve Neuroprotective Activity.” Graefe’s Archive for Clinical and Experimental Ophthalmology 248, no. 8 (August 1, 2010): 1105–16. https://doi.org/10.1007/s00417-010-1336-7.

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This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s00417-010-1336-7.

Subjects

GDNF
Retina
BDNF
Neuroprotection
CNTF

Abstract

BackgroundTo characterize the molecular and functional status of the rat retina and optic nerve after acute elevation of intraocular pressure (IOP).MethodsRetinal ischemia was induced in rats by increasing the IOP (110 mmHg/60 minutes). Microarray analysis, quantitative RT-PCR (qRT-PCR) and immunohistochemistry were used to characterize retinal tissue. PLGA microspheres containing neurotrophic factors (BDNF, GDNF, or CNTF) or empty microspheres were injected into the vitreous of operated animals 1 day after elevation of IOP. Pupil light reflex (PLR) parameters and electroretinograms (ERG) were monitored at multiple time points during the 60-day postoperative recovery period.ResultsMolecular analysis showed a significant intrinsic up-regulation of CNTF at 10 and 25 days after induction of the acute ocular hypertension (p=0.0067). Molecular tissue analysis of GDNF and its receptors (GDNFR1, GDNFR2), and BDNF and its receptor (trkB) showed no change in expression. Animals that received CNTF microspheres had no significant functional recovery compared to animals which received blank microspheres (p>0.05). Animals that received GDNF or BDNF microspheres showed significant PLR recovery (p<0.05 and p<0.001 respectively) compared to non-treated animals.ConclusionsContinuous release of neurotrophic growth factors (NGFs) significantly protects optic nerve function in the experimental model of retinal ischemia observed by PLR analysis.