Chemical Exposure-Induced Changes in the Expression of Neurotrophins and Their Receptors in the Main Olfactory System of Mice Lacking TRPM5-Expressing Microvillous Cells
dc.contributor.author | AlMatrouk, Abdullah | |
dc.contributor.author | Lemons, Kayla | |
dc.contributor.author | Ogura, Tatsuya | |
dc.contributor.author | Luo, Wangmei | |
dc.contributor.author | Wilson, Chantel | |
dc.contributor.author | Lin, Weihong | |
dc.date.accessioned | 2021-02-22T18:24:51Z | |
dc.date.available | 2021-02-22T18:24:51Z | |
dc.date.issued | 2018-09-27 | |
dc.description.abstract | Functional maintenance of the mammalian main olfactory epithelium (MOE) is challenging because of its direct exposure to a wide spectrum of environmental chemicals. We previously reported that transient receptor potential channel M5-expressing microvillous cells (TRPM5-MCs) in the MOE play an important role in olfactory maintenance. To investigate the underpinning mechanisms, we exposed transcription factor Skn-1a knockout (Skn-1a−/−) mice lacking TRPM5-MCs, and TRPM5-GFP mice to either vehicle (water) or a mixture of odorous chemicals and chitin for two weeks and analyzed the expression of olfactory signaling proteins using immunolabeling and neurotrophin (NT) and NT receptor (NTR) gene transcripts using real-time quantitative PCR. The chemical exposure did not significantly attenuate the immunolabeling of olfactory signaling proteins. Vehicle-exposed Skn-1a−/− and TRPM5-GFP mice expressed similar levels of NT and NTR gene transcripts in the MOE and olfactory bulb. Chemical exposure significantly increased MOE expression of p75NTR in Skn-1a−/− mice, while p75NTR expression was reduced in TRPM5-GFP mice, as compared to vehicle-exposed mice. Additionally, our RNA in situ hybridization analysis and immunolabeling confirmed MOE expression of most NTs and NTRs. Together, these results indicate that TRPM5-MCs and chemical exposure influence expression of some NTs and NTRs in the MOE and olfactory bulb (OB). | en_US |
dc.description.sponsorship | We thank Fenge Ni for technical assistance. We also thank Robert Margolskee and Ichiro Matsumoto for providing transgenic and knockout mice. This work was supported by a research grant (R01DC012831) to W. Lin from National Institute of Health/National Institute on Deafness and Other Communication Disorders in U.S | en_US |
dc.description.uri | https://www.mdpi.com/1422-0067/19/10/2939 | en_US |
dc.format.extent | 21 pages | en_US |
dc.genre | journal articles | en_US |
dc.identifier | doi:10.13016/m21750-uuyi | |
dc.identifier.citation | AlMatrouk, Abdullah; Lemons, Kayla; Ogura, Tatsuya; Luo, Wangmei; Wilson, Chantel; Lin, Weihong. 2018. "Chemical Exposure-Induced Changes in the Expression of Neurotrophins and Their Receptors in the Main Olfactory System of Mice Lacking TRPM5-Expressing Microvillous Cells" Int. J. Mol. Sci. 19, no. 10: 2939. https://doi.org/10.3390/ijms19102939 | en_US |
dc.identifier.uri | https://doi.org/10.3390/ijms19102939 | |
dc.identifier.uri | http://hdl.handle.net/11603/21057 | |
dc.language.iso | en_US | en_US |
dc.publisher | MDPI | en_US |
dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
dc.relation.ispartof | UMBC Biological Sciences Department Collection | |
dc.relation.ispartof | UMBC Faculty Collection | |
dc.relation.ispartof | UMBC Student Collection | |
dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Chemical Exposure-Induced Changes in the Expression of Neurotrophins and Their Receptors in the Main Olfactory System of Mice Lacking TRPM5-Expressing Microvillous Cells | en_US |
dc.title.alternative | Mice lacking transient receptor potential channel M5-expressing microvillous cells show different changes in the expression of neurotrophins and their receptors in the main olfactory system in response to chemical exposure | |
dc.type | Text | en_US |