Comprehensive Analysis of Aspergillus nidulans PKA Phosphorylome Identifies a Novel Mode of CreA Regulation

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dc.contributor.authorRibeiro, Liliane F. C.
dc.contributor.authorChelius, Cynthia
dc.contributor.authorBoppidi, Karthik R.
dc.contributor.authorNaik, Nisha S.
dc.contributor.authorHossain, Simin
dc.contributor.authorRamsey, Jessica J. J.
dc.contributor.authorKumar, Jyothi
dc.contributor.authorRibeiro, Lucas F.
dc.contributor.authorOstermeier, Marc
dc.contributor.authorTran, Bao
dc.contributor.authorGoo, Young Ah
dc.contributor.authorAssis, Leandro J. de
dc.contributor.authorUlas, Mevlut
dc.contributor.authorBayram, Ozgur
dc.contributor.authorGoldman, Gustavo H.
dc.contributor.authorLincoln, Stephen
dc.contributor.authorSrivastava, Ranjan
dc.contributor.authorHarris, Steven D.
dc.contributor.authorMarten, Mark R.
dc.date.accessioned2020-08-19T17:44:53Z
dc.date.available2020-08-19T17:44:53Z
dc.date.issued2019-04-30
dc.description.abstractThe fungal cell-wall integrity signaling (CWIS) pathway regulates cellular response to environmental stress to enable wall repair and resumption of normal growth. This complex, interconnected, pathway has been only partially characterized in filamentous fungi. To better understand the dynamic cellular response to wall perturbation, a β-glucan synthase inhibitor (micafungin) was added to a growing A. nidulans shake-flask culture. From this flask, transcriptomic and phosphoproteomic data were acquired over 10 and 120 min, respectively. To differentiate statistically-significant dynamic behavior from noise, a multivariate adaptive regression splines (MARS) model was applied to both data sets. Over 1800 genes were dynamically expressed and over 700 phosphorylation sites had changing phosphorylation levels upon micafungin exposure. Twelve kinases had altered phosphorylation and phenotypic profiling of all non-essential kinase deletion mutants revealed putative connections between PrkA, Hk-8–4, and Stk19 and the CWIS pathway. Our collective data implicate actin regulation, endocytosis, and septum formation as critical cellular processes responding to activation of the CWIS pathway, and connections between CWIS and calcium, HOG, and SIN signaling pathways.en_US
dc.description.sponsorshipThis work was supported by the National Science Foundation (Awards 1517309, 1517133, and 151690). The UNMC DNA Sequencing Core Facility receives partial support from the Nebraska Research Network In Functional Genomics NE-INBRE P20GM103427–14, The Molecular Biology of Neurosensory Systems CoBRE P30GM110768, The Fred & Pamela Buffett Cancer Center - P30CA036727, The Center for Root and Rhizobiome Innovation (CRRI) 36-5150-2085-20, and the Nebraska Research Initiative.en_US
dc.description.urihttps://journals.asm.org/doi/full/10.1128/mbio.02825-18en_US
dc.format.extent21 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2xrzt-mspj
dc.identifier.citationLiliane F. C. Ribeiro, Comprehensive Analysis of Aspergillus nidulans PKA Phosphorylome Identifies a Novel Mode of CreA Regulation, Molecular Biology and Physiology, Volume 10 Issue 2, DOI: 10.1128/mBio.02825-18en_US
dc.identifier.urihttps://doi.org/10.1128/mbio.02825-18
dc.identifier.urihttp://hdl.handle.net/11603/19462
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsAttribution 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.titleComprehensive Analysis of Aspergillus nidulans PKA Phosphorylome Identifies a Novel Mode of CreA Regulationen_US
dc.typeTexten_US

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