INFLUENCE OF AERODYNAMIC PARTICLE SIZE ON BOTULINUM NEUROTOXIN POTENCY IN MICE

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dc.contributor.advisorDabisch, Paul
dc.contributor.authorBoydston, Jeremy
dc.contributor.authorYeager, John
dc.contributor.authorTaylor, Jill
dc.contributor.authorDabisch, Paul
dc.contributor.departmentHood College Biologyen_US
dc.contributor.programBiomedical and Environmental Scienceen_US
dc.date.accessioned2020-11-13T13:01:04Z
dc.date.available2020-11-13T13:01:04Z
dc.date.issued2020-11-12
dc.description.abstractFor many agents the aerodynamic particle size can affect both the virulence and disease course in animal models. Botulinum neurotoxins (BoNTs), which are widely known as potential bioterrorism agents, are toxic via multiple routes of exposure, including small particle inhalation (1-3 μm MMAD). However, the impact of larger particle sizes on the potency of BoNT has not been previously reported. Outbred mice were exposed to BoNT-containing aerosols with differing mass median aerodynamic diameters (MMADs) of 1.1, 4.9, and 7.6 microns. Collecting aerosolized BoNT onto gelatin filters or into liquid impingers resulted in equivalent estimates of aerosol concentration. Nose-only and whole-body inhalation exposure resulted in nearly identical estimates of the median lethal dose (LD50). The LD50 for inhaled BoNT increased approximately 50-fold when the median aerodynamic particle size was increased from 1.1 to 4.9 μm, from 139 (95% CI: 111-185) to 7,324 (95% CI: 4,287-10,891) mouse intraperitoneal median lethal doses (MIPLD50).en_US
dc.description.sponsorshipThis manuscript has been authored by Battelle National Biodefense Institute, LLC under Contract No. HSHQDC-15-C-00064 with the U.S. Department of Homeland Security. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes.en_US
dc.format.extent31 pagesen_US
dc.genreThesisen_US
dc.identifierdoi:10.13016/m20pww-vedk
dc.identifier.urihttp://hdl.handle.net/11603/20045
dc.language.isoen_USen_US
dc.publisherTayor and Francis - Inhalation Toxicologyen_US
dc.relation.isAvailableAtHood College
dc.subjectBotulinum neurtoxinen_US
dc.subjectAerosolen_US
dc.subjectparticle sizeen_US
dc.subjectmiceen_US
dc.titleINFLUENCE OF AERODYNAMIC PARTICLE SIZE ON BOTULINUM NEUROTOXIN POTENCY IN MICEen_US
dc.typeTexten_US

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