Development of a Microwave—Accelerated Metal-Enhanced Fluorescence 40 Second, <100 cfu/mL Point of Care Assay for the Detection of Chlamydia Trachomatis
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2011-03
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Zhang, Yongxia, Patricia Agreda, Shannon Kelley, Charlotte Gaydos, and Chris D. Geddes. “Development of a Microwave—Accelerated Metal-Enhanced Fluorescence 40 Second, <100 Cfu/mL Point of Care Assay for the Detection of Chlamydia Trachomatis.” IEEE Transactions on Biomedical Engineering 58, no. 3 (March 2011): 781–84. https://doi.org/10.1109/TBME.2010.2066275.
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Abstract
An inexpensive technology to both lyse Chlamydia trachomatis (CT) and detect DNA released from CT within 40 s is demonstrated. In a microwave cavity, energy is highly focused using 100-nm gold films with “bow-tie” structures to lyse CT within 10 s. The ultrafast detection of the released DNA from less than 100 cfu/mL CT is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. This new “ release and detect” platform technology is a highly attractive alternative method for the lysing of bacteria, DNA extraction, and the fast quantification of bacteria and potentially other pathogenic species and cells as well. Our approach is a significant step forward for the development of a point of care test for CT.