Development of a Microwave—Accelerated Metal-Enhanced Fluorescence 40 Second, <100 cfu/mL Point of Care Assay for the Detection of Chlamydia Trachomatis

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dc.contributor.authorZhang, Yongxia
dc.contributor.authorAgreda, Patricia
dc.contributor.authorKelley, Shannon
dc.contributor.authorGaydos, Charlotte
dc.contributor.authorGeddes, Chris
dc.date.accessioned2024-10-01T18:05:34Z
dc.date.available2024-10-01T18:05:34Z
dc.date.issued2011-03
dc.description.abstractAn inexpensive technology to both lyse Chlamydia trachomatis (CT) and detect DNA released from CT within 40 s is demonstrated. In a microwave cavity, energy is highly focused using 100-nm gold films with “bow-tie” structures to lyse CT within 10 s. The ultrafast detection of the released DNA from less than 100 cfu/mL CT is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. This new “ release and detect” platform technology is a highly attractive alternative method for the lysing of bacteria, DNA extraction, and the fast quantification of bacteria and potentially other pathogenic species and cells as well. Our approach is a significant step forward for the development of a point of care test for CT.
dc.description.sponsorshipThis work was supported by the National Institute of Biomedical Imaging and Bioengineering, NIH, under Grant 1U54 EB007958-4
dc.description.urihttps://ieeexplore.ieee.org/document/5546921/
dc.format.extent11 pages
dc.genrejournal articles
dc.genrepostprints
dc.identifierdoi:10.13016/m2lbl0-u3g4
dc.identifier.citationZhang, Yongxia, Patricia Agreda, Shannon Kelley, Charlotte Gaydos, and Chris D. Geddes. “Development of a Microwave—Accelerated Metal-Enhanced Fluorescence 40 Second, <100 Cfu/mL Point of Care Assay for the Detection of Chlamydia Trachomatis.” IEEE Transactions on Biomedical Engineering 58, no. 3 (March 2011): 781–84. https://doi.org/10.1109/TBME.2010.2066275.
dc.identifier.urihttps://doi.org/10.1109/TBME.2010.2066275
dc.identifier.urihttp://hdl.handle.net/11603/36588
dc.language.isoen_US
dc.publisherIEEE
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Institute of Fluorescence (IoF)
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Chemistry & Biochemistry Department
dc.rights© 2011 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
dc.subjectGold
dc.subjectDNA
dc.subjectChlamydia trachomatis (CT)
dc.subjectAcceleration
dc.subjectlysis
dc.subjectMicrowave technology
dc.subjectTesting
dc.subjectMicroorganisms
dc.subjectElectromagnetic heating
dc.subjectMicrowave theory and techniques
dc.subjectFluorescence
dc.subjectmicrowave-accelerated metal-enhanced fluorescence (MAMEF)
dc.subjectDiseases
dc.titleDevelopment of a Microwave—Accelerated Metal-Enhanced Fluorescence 40 Second, <100 cfu/mL Point of Care Assay for the Detection of Chlamydia Trachomatis
dc.typeText
dcterms.creatorhttps://orcid.org/0000-0002-9110-6374

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