An Effective Manual Deboning Method To Prepare Intact Mouse Nasal Tissue With Preserved Anatomical Organization

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dc.contributor.authorDunston, David
dc.contributor.authorAshby, Sarah
dc.contributor.authorKrosnowski, Kurt
dc.contributor.authorOgura, Tatsuya
dc.contributor.authorLin, Weihong
dc.date.accessioned2021-02-23T16:53:28Z
dc.date.available2021-02-23T16:53:28Z
dc.date.issued2013-08-10
dc.description.abstractThe mammalian nose is a multi-functional organ with intricate internal structures. The nasal cavity is lined with various epithelia such as olfactory, respiratory, and squamous epithelia which differ markedly in anatomical locations, morphology, and functions. In adult mice, the nose is covered with various skull bones, limiting experimental access to internal structures, especially those in the posterior such as the main olfactory epithelium (MOE). Here we describe an effective method for obtaining almost the entire and intact nasal tissues with preserved anatomical organization. Using surgical tools under a dissecting microscope, we sequentially remove the skull bones surrounding the nasal tissue. This procedure can be performed on both paraformaldehyde-fixed and freshly dissected, skinned mouse heads. The entire deboning procedure takes about 20-30 min, which is significantly shorter than the experimental time required for conventional chemical-based decalcification. In addition, we present an easy method to remove air bubbles trapped between turbinates, which is critical for obtaining intact thin horizontal or coronal or sagittal sections from the nasal tissue preparation. Nasal tissue prepared using our method can be used for whole mount observation of the entire epithelia, as well as morphological, immunocytochemical, RNA in situ hybridization, and physiological studies, especially in studies where region-specific examination and comparison are of interest.en_US
dc.description.sponsorshipThis work was supported by research grants (NIH/NIDCD 009269, 012831 and ARRA administrative supplement NIH grants) to Weihong Lin. We especially thank Mr. Tim Ford at UMBC for his technical assistance in videotaping and processing. We also wish to thank Dr. Daphne Blumberg, Ms. Chere Petty at UMBC and Mr. Nicholas McCollum from Olympus America Inc. for their equipment assistance in videotaping.en_US
dc.description.urihttps://www.jove.com/t/50538/an-effective-manual-deboning-method-to-prepare-intact-mouse-nasalen_US
dc.format.extent7 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2smcy-uwmb
dc.identifier.citationDunston, D., Ashby, S., Krosnowski, K., Ogura, T., Lin, W. An Effective Manual Deboning Method To Prepare Intact Mouse Nasal Tissue With Preserved Anatomical Organization. J. Vis. Exp. (78), e50538, doi:10.3791/50538 (2013).en_US
dc.identifier.urihttps://doi.org/10.3791/50538
dc.identifier.urihttp://hdl.handle.net/11603/21064
dc.language.isoen_USen_US
dc.publisherJoVEen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rights© 2013 Journal of Visualized Experiments
dc.titleAn Effective Manual Deboning Method To Prepare Intact Mouse Nasal Tissue With Preserved Anatomical Organizationen_US
dc.typeTexten_US

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