The optimization of Naringenin biosynthesis pathway using Yarrowia lipolitica cell culture

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dc.contributor.authorMarsafari, Monireh
dc.contributor.authorLahiji, Habibollah Samizadeh
dc.contributor.authorRabiei, Babak
dc.contributor.authorMehrabi, Ali Ashraf
dc.contributor.authorLv, Yongkun
dc.contributor.authorXu, Peng
dc.date.accessioned2020-08-06T15:10:28Z
dc.date.available2020-08-06T15:10:28Z
dc.date.issued2020-06-30
dc.description.abstractYarrowia lipolytica, as a good cell factory to speed up the production of plant pharmaceutical components, has been considered to be one of the most important and attractive micro-organisms in recent years, due to its high secretion capacity, limited glycosylation, large range of genetic markers and molecular tools. Naringenin, as a central core of flavonoids production, plays important roles both in plants and in the treatment of different types of human diseases. For this purpose, specific naringenin biosynthesis genes from different origins were selected and introduced after comparative expression profiling in Y. lipolytica. This research indicated that chs plays the main role in the production of naringenin, so the increase copy number of this gene in each construct was investigated. The HPLC results confirmed that the construct with 5 copy numbers of chs resulted in 7.14 fold increase of naringenin extracellular titer to 90.16 mg/L in shake flask cultures. The results reported in this study demonstrated that sufficient knowledge of genes involved in the specific biosynthesis pathway, synthetic gene pathway and using Y. lipolytica as a capable and cheap host could help bioengineers to produce significant amounts of pharmaceutical components.en_US
dc.description.urihttps://nbr.khu.ac.ir/article-1-3298-en.htmlen_US
dc.format.extent12 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2igo4-sqyw
dc.identifier.citationMarsafari, Monireh; Lahiji, Habibollah Samizadeh; Rabiei, Babak; Mehrabi, Ali Ashraf; Lv, Yongkun; Xu, Peng; The optimization of Naringenin biosynthesis pathway using Yarrowia lipolitica cell culture; Nova Biologica Reperta 2020, 7(2): 133-144; https://nbr.khu.ac.ir/article-1-3298-en.htmlen_US
dc.identifier.urihttp://hdl.handle.net/11603/19342
dc.language.isoen_USen_US
dc.publisherKharazmi Universityen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsAttribution-NonCommercial 4.0 International (CC BY-NC 4.0)*
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/*
dc.titleThe optimization of Naringenin biosynthesis pathway using Yarrowia lipolitica cell cultureen_US
dc.typeTexten_US

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