Rapid purification of RNA secondary structures

Links to Files

https://academic.oup.com/nar/article/31/21/e135/1042627

Permanent Link

https://doi.org/10.1093/nar/gng136
 http://hdl.handle.net/11603/40624

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UMBC Faculty Collection
UMBC Chemistry & Biochemistry Department
UMBC Office of the Dean of the College of Natural and Mathematical Sciences
UMBC Student Collection

Author/Creator

Author/Creator ORCID

https://orcid.org/0000-0001-9083-6933
 https://orcid.org/0000-0003-2635-9460
 https://orcid.org/0000-0001-9214-2935

Date

2003-11-01

Type of Work

journal articles
Text

Department

Program

Citation of Original Publication

Gelhaus, Stacy L., William R. LaCourse, Nathan A. Hagan, Gaya K. Amarasinghe, and Daniele Fabris. “Rapid Purification of RNA Secondary Structures.” Nucleic Acids Research 31, no. 21 (2003): e135. https://doi.org/10.1093/nar/gng136.

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Subjects

structural analysis of oligoribonucleotides
rapid purification

Abstract

A new method for rapid purification and structural analysis of oligoribonucleotides of 19 and 20 nt is applied to RNA hairpins SL3 and SL2, which are stable secondary structures present on the ψ recognition element of HIV‐1. This approach uses ion‐pairing reversed‐phase liquid chromatography (IP‐RPLC) to achieve the separation of the stem–loop from the transcription mix. Evidence is presented that IP‐RPLC is sensitive to the different conformers of these secondary structures. The purity of each stem–loop was confirmed by mass spectrometry and PAGE. IP‐RPLC purification was found to be superior to PAGE in terms of time, safety and, most importantly, purity.