Rapid purification of RNA secondary structures

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dc.contributor.authorGelhaus, Stacy Wendell
dc.contributor.authorLaCourse, William
dc.contributor.authorHagan, Nathan
dc.contributor.authorAmarasinghe, Gaya K.
dc.contributor.authorFabris, Daniele
dc.date.accessioned2025-10-22T19:58:46Z
dc.date.issued2003-11-01
dc.description.abstractA new method for rapid purification and structural analysis of oligoribonucleotides of 19 and 20 nt is applied to RNA hairpins SL3 and SL2, which are stable secondary structures present on the ψ recognition element of HIV‐1. This approach uses ion‐pairing reversed‐phase liquid chromatography (IP‐RPLC) to achieve the separation of the stem–loop from the transcription mix. Evidence is presented that IP‐RPLC is sensitive to the different conformers of these secondary structures. The purity of each stem–loop was confirmed by mass spectrometry and PAGE. IP‐RPLC purification was found to be superior to PAGE in terms of time, safety and, most importantly, purity.
dc.description.sponsorshipWe would like to thank Dr Michael Summers, HHMI, for donating the stem±loop, SL2, for the project. We are deeply grateful for the ®nancial support from Transgenomic Inc., San Jose, CA. The SL3 synthesis and MS analysis was supported by NIH Grant R01-GM643208 (D.F.).
dc.description.urihttps://academic.oup.com/nar/article/31/21/e135/1042627
dc.format.extent6 pages
dc.genrejournal articles
dc.identifierdoi:10.13016/m2ku2d-vd2b
dc.identifier.citationGelhaus, Stacy L., William R. LaCourse, Nathan A. Hagan, Gaya K. Amarasinghe, and Daniele Fabris. “Rapid Purification of RNA Secondary Structures.” Nucleic Acids Research 31, no. 21 (2003): e135. https://doi.org/10.1093/nar/gng136.
dc.identifier.urihttps://doi.org/10.1093/nar/gng136
dc.identifier.urihttp://hdl.handle.net/11603/40624
dc.language.isoen
dc.publisherOxford University Press
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.relation.ispartofUMBC Office of the Dean of the College of Natural and Mathematical Sciences
dc.relation.ispartofUMBC Chemistry & Biochemistry Department
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectstructural analysis of oligoribonucleotides
dc.subjectrapid purification
dc.titleRapid purification of RNA secondary structures
dc.typeText
dcterms.creatorhttps://orcid.org/0000-0001-9083-6933
dcterms.creatorhttps://orcid.org/0000-0003-2635-9460
dcterms.creatorhttps://orcid.org/0000-0001-9214-2935

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