A NOVEL METHOD FOR INDUCTION OF SPECIFIC IMMUNE RESPONSE IN MICE BY ORAL IMMUNIZATION WITH Trichoplusia ni LARVAE PRODUCING A VIRUS DERIVED ANTIGEN
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Date
2015-04
Department
Hood College Graduate School
Program
Hood College Biomedical Science
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Abstract
Advances in recombinant technology led to the development of recombinant
vaccines without the drawbacks associated with the use of attenuated or inactivated live
pathogens. Baculoviruses were developed to express the capsid protein from mouse minute
virus, mouse parvovirus, mouse norovirus, and DsRed protein with varying abilities to self assemble virus like particles (VLPs). Trichoplusia ni larvae expressing these baculoviruses
were fed to mice to induce a specific immunoresponse. Successful seroconversion was
observed for MMV and MPV recombinant proteins able to self-assemble into virus like
particles. A neutralizing antibody titer was demonstrated for recombinant MMV and
MPV I . These results indicate the production of VLPs in infected larvae may be critical to
induction of a specific immune response when administered via the oral route. Thus, oral
administration of' immunogens with larvae expressing the target protein in the form of
VLPs can induce a protective immune response and may be used in vaccine development.