Nanosensors: design and application to site-specific cellular analyses

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https://www.spiedigitallibrary.org/conference-proceedings-of-spie/4615/0000/Nanosensors-design-and-application-to-site-specific-cellular-analyses/10.1117/12.466642.full

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https://doi.org/10.1117/12.466642
 http://hdl.handle.net/11603/35966

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UMBC Chemistry & Biochemistry Department

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https://orcid.org/0000-0002-5250-8290

Date

2002-05-13

Type of Work

conference papers and proceedings
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Citation of Original Publication

Cullum, Brian M., Guy D. Griffin, and Tuan Vo-Dinh. “Nanosensors: Design and Application to Site-Specific Cellular Analyses.” In Biomedical Diagnostic, Guidance, and Surgical-Assist Systems IV, 4615. (May 13, 2002):148–54. https://doi.org/10.1117/12.466642.

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THIS WORK WAS WRITTEN AS PART OF ONE OF THE AUTHOR'S OFFICIAL DUTIES AS AN EMPLOYEE OF THE UNITED STATES GOVERNMENT AND IS THEREFORE A WORK OF THE UNITED STATES GOVERNMENT. IN ACCORDANCE WITH 17 U.S.C. 105, NO COPYRIGHT PROTECTION IS AVAILABLE FOR SUCH WORKS UNDER U.S. LAW.
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Abstract

We report the development and application of an antibody-based nanoprobe for in situ measurements within a single cell. The nanoprobe has an antibody-based probe targeted to benzopyrene tetrol (BPT), a metabolite of the carcinogen benzo[a]pyrene (BaP) and the BaP-DNA adduct. Detection of BPT is of biomedical interest since this species can potentially serve as a biomarker for the monitoring of DNA damage due to BaP exposure and for possible pre-cancer diagnoses. The measurements were performed on the rat liver epithelial Clone 9 cell line, which was used as the model cell system. Nanoprobes were inserted into individual cells, incubated five minutes to allow antigen-antibody binding, and then removed for fluorescence detection. Prior to measurements, the cells had been treated with BPT. A concentration of 9.6 +/- 0.2 x 10⁻¹¹ M has been determined for BPT in the individual cells investigated. The results demonstrate the possibility of in situ measurements inside a single cell using an antibody-based nanoprobe.