Nanosensors: design and application to site-specific cellular analyses
| dc.contributor.author | Cullum, Brian | |
| dc.contributor.author | Griffin, Guy D. | |
| dc.contributor.author | Vo-Dinh, Tuan | |
| dc.date.accessioned | 2024-09-04T19:58:29Z | |
| dc.date.available | 2024-09-04T19:58:29Z | |
| dc.date.issued | 2002-05-13 | |
| dc.description | International Symposium on Biomedical Optics, 2002, San Jose, CA, United States | |
| dc.description.abstract | We report the development and application of an antibody-based nanoprobe for in situ measurements within a single cell. The nanoprobe has an antibody-based probe targeted to benzopyrene tetrol (BPT), a metabolite of the carcinogen benzo[a]pyrene (BaP) and the BaP-DNA adduct. Detection of BPT is of biomedical interest since this species can potentially serve as a biomarker for the monitoring of DNA damage due to BaP exposure and for possible pre-cancer diagnoses. The measurements were performed on the rat liver epithelial Clone 9 cell line, which was used as the model cell system. Nanoprobes were inserted into individual cells, incubated five minutes to allow antigen-antibody binding, and then removed for fluorescence detection. Prior to measurements, the cells had been treated with BPT. A concentration of 9.6 +/- 0.2 x 10⁻¹¹ M has been determined for BPT in the individual cells investigated. The results demonstrate the possibility of in situ measurements inside a single cell using an antibody-based nanoprobe. | |
| dc.description.sponsorship | This research is sponsored by the LDRD Project (Advanced Nanosensors) and by the Office of Biological and Environmental Research, U.S. Department of Energy under contract DE-AC05-000R22725 with Lockheed Martin Energy Research Corp. In addition, B. M. Cullum is also supported by an appointment to the Oak Ridge National Laboratory Postdoctoral Research Program administered jointly by the Oak Ridge Institute for Science and Education and Oak Ridge National Laboratory | |
| dc.description.uri | https://www.spiedigitallibrary.org/conference-proceedings-of-spie/4615/0000/Nanosensors-design-and-application-to-site-specific-cellular-analyses/10.1117/12.466642.full | |
| dc.format.extent | 7 pages | |
| dc.genre | conference papers and proceedings | |
| dc.identifier | doi:10.13016/m2kybb-ynug | |
| dc.identifier.citation | Cullum, Brian M., Guy D. Griffin, and Tuan Vo-Dinh. “Nanosensors: Design and Application to Site-Specific Cellular Analyses.” In Biomedical Diagnostic, Guidance, and Surgical-Assist Systems IV, 4615. (May 13, 2002):148–54. https://doi.org/10.1117/12.466642. | |
| dc.identifier.uri | https://doi.org/10.1117/12.466642 | |
| dc.identifier.uri | http://hdl.handle.net/11603/35966 | |
| dc.language.iso | en_US | |
| dc.publisher | SPIE | |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Chemistry & Biochemistry Department | |
| dc.rights | THIS WORK WAS WRITTEN AS PART OF ONE OF THE AUTHOR'S OFFICIAL DUTIES AS AN EMPLOYEE OF THE UNITED STATES GOVERNMENT AND IS THEREFORE A WORK OF THE UNITED STATES GOVERNMENT. IN ACCORDANCE WITH 17 U.S.C. 105, NO COPYRIGHT PROTECTION IS AVAILABLE FOR SUCH WORKS UNDER U.S. LAW. | |
| dc.rights | PUBLIC DOMAIN | |
| dc.rights.uri | https://creativecommons.org/publicdomain/mark/1.0/ | |
| dc.title | Nanosensors: design and application to site-specific cellular analyses | |
| dc.type | Text | |
| dcterms.creator | https://orcid.org/0000-0002-5250-8290 |
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