Mind bomb 2 promotes cell migration and epithelial structure by regulating adhesion complexes and the actin cytoskeleton

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dc.contributor.authorTrivedi, Sunny
dc.contributor.authorBhattacharya, Mallika
dc.contributor.authorStarz-Gaiano, Michelle
dc.date.accessioned2022-10-17T15:57:48Z
dc.date.available2022-10-17T15:57:48Z
dc.date.issued2022-09-17
dc.description.abstractCell migration is essential in animal development and co-opted during metastasis and inflammatory diseases. Some cells migrate collectively, which requires them to balance epithelial characteristics such as stable cell-cell adhesions with features of motility like rapid turnover of adhesions and dynamic cytoskeletal structures. How this is regulated is not entirely clear but important to understand. While investigating Drosophila oogenesis, we found that the putative E3 ubiquitin ligase, Mind bomb 2 (Mib2), is required to promote epithelial stability and the collective cell migration of border cells. Through biochemical analysis, we identified components of Mib2 complexes, which include E-cadherin and α- and β-catenins, as well as actin regulators. We also found that three Mib2 interacting proteins, RhoGAP19D, Supervillin, and Myosin heavy chain-like, affect border cell migration. mib2 mutant main body follicle cells have drastically reduced E-cadherin-based adhesion complexes and diminished actin filaments. We conclude that Mib2 acts to stabilize E-cadherin-based adhesion complexes and promote a robust actin cytoskeletal network, which is important for maintenance of epithelial integrity. The interaction with cadherin adhesion complexes and other cytoskeletal regulators contribute to its role in collective cell migration. Since Mib2 is well conserved, it may have similar functional significance in other organisms.en_US
dc.description.sponsorshipThis work was supported in part by the National Science Foundation [grant IOS-1656550 to MSG].en_US
dc.description.urihttps://www.sciencedirect.com/science/article/pii/S0012160622001610?via%3Dihuben_US
dc.format.extent38 pagesen_US
dc.genrejournal articlesen_US
dc.genrepostprintsen_US
dc.identifierdoi:10.13016/m2lffa-cz1r
dc.identifier.citationTrivedi, Sunny; Mallika Bhattacharya and Michelle Starz-Gaiano." Mind bomb 2 promotes cell migration and epithelial structure by regulating adhesion complexes and the actin cytoskeleton." Developmental Biology 491 (17 September 2022): 94-104. https://doi.org/10.1016/j.ydbio.2022.08.009.en_US
dc.identifier.urihttps://doi.org/10.1016/j.ydbio.2022.08.009
dc.identifier.urihttp://hdl.handle.net/11603/26191
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.en_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)*
dc.rightsAccess to this item will begin on 09-17-2023
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleMind bomb 2 promotes cell migration and epithelial structure by regulating adhesion complexes and the actin cytoskeletonen_US
dc.title.alternativeMind bomb 2 stabilizes E-cadherin and the actin cytoskeleton to promote epithelial organization and cell migration in Drosophilaen_US
dc.title.alternativeMib2 regulates cytoskeletonen_US
dc.typeTexten_US
dcterms.creatorhttps://orcid.org/0000-0002-9840-9028en_US
dcterms.creatorhttps://orcid.org/0000-0002-0855-0715en_US

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