Systematic overexpression of genes encoded by mycobacteriophage Waterfoul reveals novel inhibitors of mycobacterial growth

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dc.contributor.authorHeller, Danielle
dc.contributor.authorAmaya, Isabel
dc.contributor.authorMohamed, Aleem
dc.contributor.authorAli, Ilzat
dc.contributor.authorMavrodi, Dmitri
dc.contributor.authorDeighan, Padraig
dc.contributor.authorSivanathan, Viknesh
dc.date.accessioned2022-07-14T18:54:37Z
dc.date.available2022-07-14T18:54:37Z
dc.date.issued2022-06-21
dc.description.abstractBacteriophages represent an enormous reservoir of novel genes, many of which are unrelated to existing entries in public databases and cannot be assigned a predicted function. Characterization of these genes can provide important insights into the intricacies of phage–host interactions and may offer new strategies to manipulate bacterial growth and behavior. Overexpression is a useful tool in the study of gene-mediated effects, and we describe here the construction of a plasmid-based overexpression library of a complete set of genes for Waterfoul, a mycobacteriophage closely related to those infecting clinically important strains of Mycobacterium tuberculosis and/or Mycobacterium abscessus. The arrayed Waterfoul gene library was systematically screened in a plate-based cytotoxicity assay, identifying a diverse set of 32 Waterfoul gene products capable of inhibiting the growth of the host Mycobacterium smegmatis and providing a first look at the frequency and distribution of cytotoxic products encoded within a single mycobacteriophage genome. Several of these Waterfoul gene products were observed to confer potent anti-mycobacterial effects, making them interesting candidates for follow-up mechanistic studies.en_US
dc.description.sponsorshipThis work was conducted as part of the HHMI-supported Science Education Alliance GENES (Gene function Exploration by a Network of Emerging Scientists) project.en_US
dc.description.urihttps://academic.oup.com/g3journal/advance-article/doi/10.1093/g3journal/jkac140/6612092en_US
dc.format.extent8 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m28rle-qdpi
dc.identifier.citationDanielle Heller, Isabel Amaya, Aleem Mohamed, Ilzat Ali, Dmitri Mavrodi, Padraig Deighan, Viknesh Sivanathan, Systematic overexpression of genes encoded by mycobacteriophage Waterfoul reveals novel inhibitors of mycobacterial growth, G3 Genes|Genomes|Genetics, 2022;, jkac140, https://doi.org/10.1093/g3journal/jkac140en_US
dc.identifier.urihttps://doi.org/10.1093/g3journal/jkac140
dc.identifier.urihttp://hdl.handle.net/11603/25151
dc.language.isoen_USen_US
dc.publisherOxforden_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Student Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.titleSystematic overexpression of genes encoded by mycobacteriophage Waterfoul reveals novel inhibitors of mycobacterial growthen_US
dc.typeTexten_US
dcterms.creatorhttps://orcid.org/0000-0003-2019-5645en_US

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