Function-adaptive clustered nanoparticles reverse Streptococcus mutans dental biofilm and maintain microbiota balance

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dc.contributor.authorOstadhossein, Fatemeh
dc.contributor.authorMoitra, Parikshit
dc.contributor.authorAltun, Esra
dc.contributor.authorDutta, Debapriya
dc.contributor.authorSar, Dinabandhu
dc.contributor.authorTripathi, Indu
dc.contributor.authorHsiao, Shih-Hsuan
dc.contributor.authorKravchuk, Valeriya
dc.contributor.authorNie, Shuming
dc.contributor.authorPan, Dipanjan
dc.date.accessioned2021-08-05T14:51:31Z
dc.date.available2021-08-05T14:51:31Z
dc.date.issued2021-07-15
dc.description.abstractDental plaques are biofilms that cause dental caries by demineralization with acidogenic bacteria. These bacteria reside inside a protective sheath which makes any curative treatment challenging. We propose an antibiotic-free strategy to disrupt the biofilm by engineered clustered carbon dot nanoparticles that function in the acidic environment of the biofilms. In vitro and ex vivo studies on the mature biofilms of Streptococcus mutans revealed >90% biofilm inhibition associated with the contact-mediated interaction of nanoparticles with the bacterial membrane, excessive reactive oxygen species generation, and DNA fragmentation. An in vivo examination showed that these nanoparticles could effectively suppress the growth of S. mutans. Importantly, 16S rRNA analysis of the dental microbiota showed that the diversity and richness of bacterial species did not substantially change with nanoparticle treatment. Overall, this study presents a safe and effective approach to decrease the dental biofilm formation without disrupting the ecological balance of the oral cavity.en_US
dc.description.sponsorshipWe thank the staff of the Frederick Seitz Materials Research Laboratory, the Roger Adams NMR Lab (Dr. Andre Sutrisno), and the Beckman Institute of Technology Microscopic Suite (Mrs. Catherine Wallace and Mr. Scott J Robinson) for their experimental and technical support. FTIR studies were conducted at the Frederick Seitz Materials Research Laboratory, UIUC. The NMR studies were performed at the Roger Adams Laboratory, UIUC. Animal studies, TEM, SEM, and confocal imaging were performed at the Beckman Research Institute. We thank Ms. Renee Walker from VetMed and Dr. Kingsley Boateng for the preparation of histology slides, and help with the corresponding experiments. We appreciate the help received from Dr. Richard Haasch for the XPS study. We also appreciate Maha Alafeef and Ketan Dighe for providing the S. mutans culture for the experimental studies during revision. We acknowledge financial support from the University of Illinois at Urbana-Champaign, National Institute of Health, Department of Defense and the Children’s Discovery Institute. F.O. is supported by American Heart Association grant #18pre34080003/2018 and a postdoctoral fellowship from the Beckman Institute. This manuscript has been proof-read by Nature Research Editing Service for grammar and command of language.en_US
dc.description.urihttps://www.nature.com/articles/s42003-021-02372-yen_US
dc.format.extent5 filesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2qxs2-jqio
dc.identifier.citationOstadhossein, Fatemeh et al.; Function-adaptive clustered nanoparticles reverse Streptococcus mutans dental biofilm and maintain microbiota balance; Communications Biology, volume 4, Article number: 846, 15 July, 2021; https://doi.org/10.1038/s42003-021-02372-yen_US
dc.identifier.urihttps://doi.org/10.1038/s42003-021-02372-y
dc.identifier.urihttp://hdl.handle.net/11603/22307
dc.language.isoen_USen_US
dc.publisherNatureen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.titleFunction-adaptive clustered nanoparticles reverse Streptococcus mutans dental biofilm and maintain microbiota balanceen_US
dc.typeTexten_US
dcterms.creatorhttps://orcid.org/0000-0003-0175-4704en_US

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