Assessing Fab-Functionalized Gold Nanoparticles-Mediated Thermal Enhancement during High-Intensity Focused Ultrasound Ablation in a Mouse Tumor Model

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https://pubs.acs.org/doi/full/10.1021/acsabm.5c00879

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https://doi.org/10.1021/acsabm.5c00879
 http://hdl.handle.net/11603/40887

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UMBC Mechanical Engineering Department
UMBC Chemistry & Biochemistry Department
UMBC Faculty Collection
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Author/Creator ORCID

https://orcid.org/0000-0002-7336-5655

Date

2025-10-13

Type of Work

journal articles
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Citation of Original Publication

Khanal, Nabin, Michael A. Marciniak, Marie-Christine Daniel, et al. “Assessing Fab-Functionalized Gold Nanoparticles-Mediated Thermal Enhancement during High-Intensity Focused Ultrasound Ablation in a Mouse Tumor Model.” ACS Applied Bio Materials, American Chemical Society, October 13, 2025. https://doi.org/10.1021/acsabm.5c00879.

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This work was written as part of one of the author's official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law.
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UMBC Daniel Lab

Abstract

High-intensity focused ultrasound (HIFU) stands out as a noninvasive modality that is gaining prominence for the localized treatment of malignant tumors. A mouse tumor model was used to assess the level of thermal enhancement afforded by Fab-functionalized gold nanoparticles (gNPs) during HIFU treatment. Prostate cancer cells (PC3) were used to grow tumors on the right flank of immunodeficient NSG mice. Three levels of gNPs concentrations (0%, 0.019%, and 0.125%) were injected directly into the tumors. HIFU sonication was performed at acoustic power levels of 30W, 40W, and 50W for the duration of 16 s inside a 1.5 T magnetic resonance system. Temperature rise data were recorded for each power level and gNPs concentration during the experiment and analyzed. Tumors were harvested 4 h after the sonication for a histopathology study. A histopathology study was conducted using hematoxylin and eosin (H&E) as well as cleaved caspase 3 (CC3) staining. For an acoustic power of 50W, temperature increases of 16.77 ± 2.33 °C, 19.95 ± 2.98 °C, and 27.78 ± 5.31 °C were recorded for gNPs concentrations of 0%, 0.019%, and 0.125%, respectively. Also, for an acoustic power of 50W, thermal doses of 0.08, 282.87, and 31563.70 min were obtained for gNPs concentrations of 0%, 0.019%, and 0.125%, respectively. Cellular damage around the focus was observed in histopathology studies using H&E staining in HIFU-treated tumors.