Chemotaxis of Drosophila Border Cells is Modulated by Tissue Geometry Through Dispersion of Chemoattractants
dc.contributor.author | George, Alexander | |
dc.contributor.author | Akhavan, Naghmeh | |
dc.contributor.author | Peercy, Bradford | |
dc.contributor.author | Starz-Gaiano, Michelle | |
dc.date.accessioned | 2025-03-11T14:42:30Z | |
dc.date.available | 2025-03-11T14:42:30Z | |
dc.date.issued | 2025-02-05 | |
dc.description.abstract | Migratory cells respond to graded concentrations of diffusible chemoattractants in vitro, but how complex tissue geometries in vivo impact chemotaxis is poorly understood. To address this, we studied the Drosophila border cells. Live-imaged border cells varied in their chemotactic migration speeds, which correlated positionally with distinct architectures. We then developed a reduced mathematical model to determine how chemoattractant distribution is affected by tissue architecture. Larger extracellular volumes locally dampened the chemoattractant gradient and, when coupled with an agent-based motion of the cluster, reduced cell speeds. This suggests that chemoattractant levels vary by tissue architectures, informing cell migration behaviors locally, which we tested in vivo. Genetically elevating chemoattractant levels slowed migration in specific architectural regions, while mutants with spacious tissue structure rescued defects from high chemoattractant levels, promoting punctual migration. Our results highlight the interplay between tissue geometry and the local distribution of signaling molecules to orchestrate cell migration. | |
dc.description.sponsorship | We acknowledge funding from NSF DMS #1953423 to B.E.P. and M.S.-G., NSF IOS #2303587 to M.S.-G. and MERCK SHARP and DOHM LLC funding for N.A. For providing commercial fly stocks, we thank the Bloomington Drosophila Stock Center (NIH P40OD018537) and we thank Dr. Trudi Dr. Schupbach for gifting us critical mutant fly lines. We thank FlyBase (release € FB2024_05)56 for all their resources | |
dc.description.uri | https://www.sciencedirect.com/science/article/pii/S2589004225002196 | |
dc.format.extent | 20 pages | |
dc.genre | journal articles | |
dc.identifier | doi:10.13016/m2tbb6-zvnt | |
dc.identifier.citation | George, Alexander, Naghmeh Akhavan, Bradford E. Peercy, and Michelle Starz-Gaiano. "Chemotaxis of Drosophila Border Cells Is Modulated by Tissue Geometry Through Dispersion of Chemoattractants". iScience. February 5, 2025, 111959. https://doi.org/10.1016/j.isci.2025.111959. | |
dc.identifier.uri | https://doi.org/10.1016/j.isci.2025.111959 | |
dc.identifier.uri | http://hdl.handle.net/11603/37740 | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | |
dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
dc.relation.ispartof | UMBC Biological Sciences Department | |
dc.relation.ispartof | UMBC Student Collection | |
dc.relation.ispartof | UMBC Mathematics and Statistics Department | |
dc.relation.ispartof | UMBC Faculty Collection | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | chemoattractant | |
dc.subject | Drosophila border cell migration | |
dc.subject | chemotaxis | |
dc.subject | mathematical modeling | |
dc.subject | growth factor signaling | |
dc.subject | three-dimensional migration | |
dc.subject | Drosophila oogenesis | |
dc.subject | collective cell migration | |
dc.title | Chemotaxis of Drosophila Border Cells is Modulated by Tissue Geometry Through Dispersion of Chemoattractants | |
dc.type | Text | |
dcterms.creator | https://orcid.org/0000-0002-9474-4486 | |
dcterms.creator | https://orcid.org/0000-0002-8597-2508 | |
dcterms.creator | https://orcid.org/0000-0002-0855-0715 |
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