Development of a protein microarray for analysis of humoral immune responses to flavivirus infection and vaccination

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dc.contributorKeasey, Sarah
dc.contributorSmith, Jessica
dc.contributorJensen, Stig
dc.contributor.advisorUlrich, Robert
dc.contributor.advisorLaufer, Craig
dc.contributor.advisorFernandez, Stefan
dc.contributor.authorPugh, Christine
dc.contributor.departmentGraduate department-Biologyen_US
dc.contributor.programBiomedical Science (M.S.)en_US
dc.date.accessioned2018-04-16T12:32:46Z
dc.date.available2018-04-16T12:32:46Z
dc.date.issued2018-04-13
dc.descriptionEMBARGO 1 YEAR 4/19/2018 Part of this thesis was previously published (Feb. 2017) in Clinical and Vaccine Immunology with the title: Antibody response to Zika virus infections in environments of flavivirus endemicity. Citation:Keasey SL, Pugh CL, Jensen SMR, Smith JL, Hontz RD, Durbin AP, Dudley DM, O'Connor DH, Ulrich RG. 2017. Antibody responses to Zika virus infections in environments of flavivirus endemicity. Clin Vaccine Immunol 24:e00036-17. https://doi.org/10.1128/CVI.00036-17.en_US
dc.description.abstractDengue virus (DENV), yellow fever virus (YFV), West Nile virus (WNV), and Zika virus (ZIKV) are the leading causes of mosquito-borne human flavivirus infections in the Americas. Serological assays for diagnosis and surveillance are generally directed towards one specific virus and disregard the potential for antibody cross reactivity between nearest neighbors. Here, a comprehensive assay was developed that included whole virus preparations and recombinant antigens from 15 human pathogenic flaviviruses. Using the printed microarrays, serological immune responses to ZIKV, WNV, DENV, and YFV infections of humans and nonhuman primates (NHPs) were examined in order to examine specificity and cross reactivity of antibody responses among the viral antigens. Sera were further employed from yellow fever vaccine studies to demonstrate the utility of using multiple viral antigens for obtaining a detailed analysis of antibody responses to vaccination. Results from the microarray assays indicated that antibody recognition of isolated flaviviral antigens can be used to resolve complex infection histories, as well as provide a detailed understanding of immune responses to YFV vaccination in areas of flavivirus endemicity.en_US
dc.description.sponsorshipThis research was supported in part by an appointment to the Postgraduate Research Participation Program administered by Oak Ridge Institute for Science and Education through an interagency agreement with the U.S. Department of Energy.en_US
dc.genrethesesen_US
dc.identifierdoi:10.13016/M2PN8XH5R
dc.identifier.urihttp://hdl.handle.net/11603/8763
dc.language.isoen_USen_US
dc.relation.isAvailableAtHood College
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectflavivirusen_US
dc.subjecthumoral immunityen_US
dc.subjectcross-reactivityen_US
dc.subjectvaccinationen_US
dc.subjectcross-reactivityen_US
dc.titleDevelopment of a protein microarray for analysis of humoral immune responses to flavivirus infection and vaccinationen_US
dc.typeCollectionen_US

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