Identification and Characterization of VpsR and VpsT Binding Sites in Vibrio cholerae

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dc.contributor.authorZamorano-Sánchez, David
dc.contributor.authorFong, Jiunn C. N.
dc.contributor.authorKilic, Sefa
dc.contributor.authorErill, Ivan
dc.contributor.authorYildiz, Fitnat H.
dc.date.accessioned2021-03-08T18:18:59Z
dc.date.available2021-03-08T18:18:59Z
dc.date.issued2015-01-26
dc.description.abstractThe ability to form biofilms is critical for environmental survival and transmission of Vibrio cholerae, a facultative human pathogen responsible for the disease cholera. Biofilm formation is controlled by several transcriptional regulators and alternative sigma factors. In this study, we report that the two main positive regulators of biofilm formation, VpsR and VpsT, bind to nonoverlapping target sequences in the regulatory region of vpsL in vitro. VpsR binds to a proximal site (the R1 box) as well as a distal site (the R2 box) with respect to the transcriptional start site identified upstream of vpsL. The VpsT binding site (the T box) is located between the R1 and R2 boxes. While mutations in the T and R boxes resulted in a decrease in vpsL expression, deletion of the T and R2 boxes resulted in an increase in vpsL expression. Analysis of the role of H-NS in vpsL expression revealed that deletion of hns resulted in enhanced vpsL expression. The level of vpsL expression was higher in an hns vpsT double mutant than in the parental strain but lower than that in an hns mutant. In silico analysis of the regulatory regions of the VpsR and VpsT targets resulted in the identification of conserved recognition motifs for VpsR and VpsT and revealed that operons involved in biofilm formation and vpsT are coregulated by VpsR and VpsT. Furthermore, a comparative genomics analysis revealed substantial variability in the promoter region of the vpsT and vpsL genes among extant V. cholerae isolates, suggesting that regulation of biofilm formation is under active selection.en_US
dc.description.sponsorshipThis work was supported by NIH grant R01AI055987 (to F.H.Y.) and by U.S. National Science Foundation grant MCB-1158056 (to I.E.). D.Z.-S. was supported by a UC-MEXUS fellowship. We thank Jennifer Teschler and Jenna Conner for their comments on the manuscript.en_US
dc.description.urihttps://jb.asm.org/content/197/7/1221.longen_US
dc.format.extent15 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2osgw-546d
dc.identifier.citationZamorano-Sánchez D, Fong JCN, Kilic S, Erill I, Yildiz FH. 2015. Identification and characterization of VpsR and VpsT binding sites in Vibrio cholerae. J Bacteriol 197:1221–1235. doi:10.1128/JB.02439-14.en_US
dc.identifier.urihttps://doi.org/10.1128/JB.02439-14.
dc.identifier.urihttp://hdl.handle.net/11603/21093
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectcholeraen_US
dc.subjectVpsRen_US
dc.subjectVpsTen_US
dc.subjectDNA manipulationsen_US
dc.titleIdentification and Characterization of VpsR and VpsT Binding Sites in Vibrio choleraeen_US
dc.typeTexten_US

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