Metal-enhanced bioluminescence: An approach for monitoring biological luminescent processes
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Eltzov, Evgeni, Daria Prilutsky, Ariel Kushmaro, Robert S. Marks, and Chris D. Geddes. “Metal-Enhanced Bioluminescence: An Approach for Monitoring Biological Luminescent Processes.” Applied Physics Letters 94, no. 8 (February 23, 2009): 083901. https://doi.org/10.1063/1.3086283.
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This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. This article appeared in Eltzov, Evgeni, Daria Prilutsky, Ariel Kushmaro, Robert S. Marks, and Chris D. Geddes. “Metal-Enhanced Bioluminescence: An Approach for Monitoring Biological Luminescent Processes.” Applied Physics Letters 94, no. 8 (February 23, 2009): 083901. https://doi.org/10.1063/1.3086283 and may be found at https://pubs.aip.org/aip/apl/article/94/8/083901/337423/Metal-enhanced-bioluminescence-An-approach-for.
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Abstract
In this letter, the observation of metal (plasmon)-enhanced bioluminescence is reported. Bacteria, which are capable of generating specific bioluminescence signatures upon metabolic changes (general toxicity), have been studied from both glass and silvered glass microwell bottoms, where the silvered microwells have been modified with surface deposited silver island films (SiFs). The presence of the SiFs plasmon amplifies the near-field bioluminescence signatures, ≈<50nm from the surface, enabling amplified detection of the reporter bioluminescence indicating sample toxicity. Using our approach a greater than fivefold enhancement in far-field bioluminescence occurs with much greater enhancements in the near-field predicted.