Primate Retroviruses: Biochemical Characterization Based on the Isoelectric Points of the Envelope Glycoproteins
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Hood College Biology
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Biomedical and Environmental Science
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Abstract
The major envelope glycoproteins of Rauscher murine leukemia virus, feline leukemia virus, RD114, baboon virus, woolly monkey virus, Mason Pfizer monkey virus, and gibbon ape whole virus preparations were examined by isoelectric focusing and competition immunoassay for their isoelectric points. All exhibited isoelectric points in the range of 3.92 to 7.32. All, except feline leukemia virus, exhibited two separate isoelectric points designated Peak I and Peak II. Peak I had isoelectric points in the range of 3.92 to 5.35. Peak II had isoelectric points in the range of 5.35 to 7.32. Isoelectric focusing of purified baboon virus 70,000 molecular weight glycoprotein (gp70) demonstrated the absence of Peak II as seen in the whole virus focusing of baboon. This is the first evidence of the possibility of a lower molecular weight glycosylated breakdown product of gp70 in a virus isolate other than Rauscher murine leukemia virus.