ANALYSIS OF A RECOMBINANT MONOCLONAL ANTIBODY BY MOLECULAR SIEVING CAPILLARY ELECTROPHORESIS
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Hood College Biology
Biomedical and Environmental Science
Citation of Original Publication
Research reported in this document was originally published in a Master's degree thesis sponsored by Human Genome Sciences Inc., Rockville, Maryland and Department of Biology, Hood College, Frederick, Maryland, and submitted to The Graduate School of Hood College.
The rising interest in the use of mAb therapeutics against human diseases has led to the development of more sophisticated analytical methods to detect and quantitate size variants. Although SDS-CE using gel or non-gel based polymer matrices for size-based separation of SDS-protein complexes has the potential to replace SDS-PAGE due to its numerous advantages, more extensive application data is needed. This study investigated the utility of non-gel polymer solutions of PEG, PEO and dextran as matrices for the separation of SDS-mAb complexes in a size-dependent manner in fused-silica capillaries. The 5-10% PEG and 3% PEO solutions unsuitable for use as separation matrices in barefused silica capillaries worked well in the neutral fused silica capillary. The 7.5 and 10% dextran solutions were suitable for use as separation matrices in both bare and neutral fused silica capillaries. The separations on the neutral fused silica capillary proved superior to corresponding separations performed on the bare-fused silica capillary.