ANALYSIS OF A RECOMBINANT MONOCLONAL ANTIBODY BY MOLECULAR SIEVING CAPILLARY ELECTROPHORESIS
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Author/Creator ORCID
Date
2006
Department
Hood College Biology
Program
Biomedical and Environmental Science
Citation of Original Publication
Rights
Research reported in this document was originally published in a Master's degree thesis
sponsored by Human Genome Sciences Inc., Rockville, Maryland and Department of
Biology, Hood College, Frederick, Maryland, and submitted to The Graduate School of
Hood College.
Subjects
Abstract
The rising interest in the use of mAb therapeutics against human diseases has led to the
development of more sophisticated analytical methods to detect and quantitate size
variants. Although SDS-CE using gel or non-gel based polymer matrices for size-based
separation of SDS-protein complexes has the potential to replace SDS-PAGE due to its
numerous advantages, more extensive application data is needed. This study investigated
the utility of non-gel polymer solutions of PEG, PEO and dextran as matrices for the
separation of SDS-mAb complexes in a size-dependent manner in fused-silica capillaries.
The 5-10% PEG and 3% PEO solutions unsuitable for use as separation matrices in barefused
silica capillaries worked well in the neutral fused silica capillary. The 7.5 and 10%
dextran solutions were suitable for use as separation matrices in both bare and neutral
fused silica capillaries. The separations on the neutral fused silica capillary proved
superior to corresponding separations performed on the bare-fused silica capillary.