Blind Evaluation of the Microwave-Accelerated Metal-Enhanced Fluorescence Ultrarapid and Sensitive Chlamydia trachomatis Test by Use of Clinical Samples

dc.contributor.authorMelendez, Johan
dc.contributor.authorHuppert, Jill S.
dc.contributor.authorJett-Goheen, Mary
dc.contributor.authorHesse, Elizabeth A.
dc.contributor.authorQuinn, Nicole
dc.contributor.authorGaydos, Charlotte A.
dc.contributor.authorGeddes, Chris
dc.date.accessioned2024-10-01T18:05:31Z
dc.date.available2024-10-01T18:05:31Z
dc.date.issued2020-12-21
dc.description.abstractAccurate point-of-care (POC) diagnostic tests for Chlamydia trachomatis infection are urgently needed for the rapid treatment of patients. In a blind comparative study, we evaluated microwave-accelerated metal-enhanced fluorescence (MAMEF) assays for ultrafast and sensitive detection of C. trachomatis DNA from vaginal swabs. The results of two distinct MAMEF assays were compared to those of nucleic acid amplification tests (NAATs). The first assay targeted the C. trachomatis 16S rRNA gene, and the second assay targeted the C. trachomatis cryptic plasmid. Using pure C. trachomatis, the MAMEF assays detected as few as 10 inclusion-forming units/ml of C. trachomatis in less than 9 min, including DNA extraction and detection. A total of 257 dry vaginal swabs from 245 female adolescents aged 14 to 22 years were analyzed. Swabs were eluted with water, the solutions were lysed to release and to fragment genomic DNA, and MAMEF-based DNA detection was performed. The prevalence of C. trachomatis by NAATs was 17.5%. Of the 45 samples that were C. trachomatis positive and the 212 samples that were C. trachomatis negative by NAATs, 33/45 and 197/212 were correctly identified by the MAMEF assays if both assays were required to be positive (sensitivity, 73.3%; specificity, 92.9%). Using the plasmid-based assay alone, 37/45 C. trachomatis-positive and 197/212 C. trachomatis-negative samples were detected (sensitivity, 82.2%; specificity, 92.9%). Using the 16S rRNA assay alone, 34/45 C. trachomatis-positive and 197/212 C. trachomatis-negative samples were detected (sensitivity, 75.5%; specificity, 92.9%). The overall rates of agreement with NAAT results for the individual 16S rRNA and cryptic plasmid assays were 89.5% and 91.0%, respectively. Given the sensitivity, specificity, and rapid detection of the plasmid-based assay, the plasmid-based MAMEF assay appears to be suited for clinical POC testing.
dc.description.sponsorshipWe acknowledge the NIH for support (NIBIB grant U54 EB007958) and acknowledge salary support from the Institute of Fluorescence, the Department of Chemistry and Biochemistry, and the University of Maryland Baltimore County to both C.D.G. and J.H.M. Training support for J.H.M. also was provided by NIGMS grant R25 GM55036 and NIH grant T32 GM66706.
dc.description.urihttps://journals.asm.org/doi/10.1128/jcm.00980-13
dc.format.extent8 pages
dc.genrejournal articles
dc.identifierdoi:10.13016/m2p7uu-3dv6
dc.identifier.citationMelendez, Johan H., Jill S. Huppert, Mary Jett-Goheen, Elizabeth A. Hesse, Nicole Quinn, Charlotte A. Gaydos, and Chris D. Geddes. “Blind Evaluation of the Microwave-Accelerated Metal-Enhanced Fluorescence Ultrarapid and Sensitive Chlamydia Trachomatis Test by Use of Clinical Samples.” Journal of Clinical Microbiology 51, no. 9 (December 21, 2020): 2913–20. https://doi.org/10.1128/jcm.00980-13.
dc.identifier.urihttps://doi.org/10.1128/jcm.00980-13
dc.identifier.urihttp://hdl.handle.net/11603/36582
dc.language.isoen_US
dc.publisherASM
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Institute of Fluorescence (IoF)
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Chemistry & Biochemistry Department
dc.relation.ispartofUMBC Student Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.titleBlind Evaluation of the Microwave-Accelerated Metal-Enhanced Fluorescence Ultrarapid and Sensitive Chlamydia trachomatis Test by Use of Clinical Samples
dc.typeText
dcterms.creatorhttps://orcid.org/0000-0002-9110-6374

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