Microfibrous Extracellular Matrix Changes the Liver Hepatocyte Energy Metabolism via Integrins

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dc.contributor.authorHuang, Tianjiao
dc.contributor.authorJones, Curtis G.
dc.contributor.authorChung, Jay H.
dc.contributor.authorChen, Chengpeng
dc.date.accessioned2021-06-24T20:41:40Z
dc.date.available2021-06-24T20:41:40Z
dc.date.issued2020-09-17
dc.description.abstractCell line-based liver models are critical tools for liver-related studies. However, the conventional monolayer culture of hepatocytes, the most widely used in vitro model, does not have the extracellular matrix (ECM), which contributes to the three-dimensional (3D) arrangement of the hepatocytes in the liver. As a result, the metabolic properties of the hepatocytes in the monolayer tissue culture may not accurately reflect those of the hepatocytes in the liver. Here, we developed a modular platform for 3D hepatocyte cultures on fibrous ECMs produced by electrospinning, a technique that can turn a polymer solution to the micro/nanofibers and has been widely used to produce scaffolds for 3D cell cultures. Metabolomics quantitation by liquid chromatography–mass spectrometry (LC–MS) indicated that Huh7 hepatocytes grown in microfibers electrospun from silk fibroin exhibited reduced glycolysis and tricarboxylic acid (TCA) cycle, as compared to the cells cultured as a monolayer. Further mechanistic studies suggested that integrins were correlated to the ECM’s effects. This is the first time to report how an ECM scaffold could affect the fundamental metabolism of the hepatocytes via integrins.en_US
dc.description.sponsorshipThis work was supported by the startup funding of UMBC and the Intramural Research Program of National Heart Lung and Blood Institute, National Institutes of Health. We thank the NHLBI Biochemistry Core and Dr. Duck-Yeon Lee’s assistance for Mass Spectrometry and the Keith Porter Imaging Center at UMBC.en_US
dc.description.urihttps://pubs.acs.org/doi/10.1021/acsbiomaterials.0c01311en_US
dc.format.extent2 filesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m27hht-vpf4
dc.identifier.citationHuang, Tianjiao et al; Microfibrous Extracellular Matrix Changes the Liver Hepatocyte Energy Metabolism via Integrins; ACS Biomaterials Science and Enginering 6,10, 5849-5856, 17 September, 2020; https://doi.org/10.1021/acsbiomaterials.0c01311en_US
dc.identifier.urihttps://doi.org/10.1021/acsbiomaterials.0c01311
dc.identifier.urihttp://hdl.handle.net/11603/21822
dc.language.isoen_USen_US
dc.publisherACS Publicationsen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemistry & Biochemistry Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.relation.ispartofUMBC Student Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsPublic Domain Mark 1.0*
dc.rightsThis work was written as part of one of the author's official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law.
dc.rights.urihttp://creativecommons.org/publicdomain/mark/1.0/*
dc.titleMicrofibrous Extracellular Matrix Changes the Liver Hepatocyte Energy Metabolism via Integrinsen_US
dc.typeTexten_US

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