Cell-free protein synthesis: advances on production process for biopharmaceuticals and immunobiological products
Loading...
Links to Files
Author/Creator ORCID
Date
2021-01-20
Type of Work
Department
Program
Citation of Original Publication
Chiba, Camila Hiromi; Knirsch, Marcos Camargo; Azzoni, Adriano Rodrigues; Moreira, Antonio R; Stephano, Marco Antonio; Cell-free protein synthesis: advances on production process for biopharmaceuticals and immunobiological products; BioTechniques 70,2 (2021); https://www.future-science.com/doi/10.2144/btn-2020-0155
Rights
This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Subjects
Abstract
Biopharmaceutical products are of great importance in the treatment or prevention of many diseases and represent a growing share of the global pharmaceutical market. The usual technology for protein synthesis (cell-based expression) faces certain obstacles, especially with ‘difficult-to-express’ proteins. Cell-free protein synthesis (CFPS) can overcome the main bottlenecks of cell-based expression. This review aims to present recent advances in the production process of biologic products by CFPS. First, key aspects of CFPS systems are summarized. A description of several biologic products that have been successfully produced using the CFPS system is provided. Finally, the CFPS system's ability to scale up and scale down, its main limitations and its application for biologics production are discussed.