Cell-free protein synthesis: advances on production process for biopharmaceuticals and immunobiological products
| dc.contributor.author | Chiba, Camila Hiromi | |
| dc.contributor.author | Knirsch, Marcos Camargo | |
| dc.contributor.author | Azzoni, Adriano Rodrigues | |
| dc.contributor.author | Moreira, Antonio R | |
| dc.contributor.author | Stephano, Marco Antonio | |
| dc.date.accessioned | 2021-02-16T16:51:55Z | |
| dc.date.available | 2021-02-16T16:51:55Z | |
| dc.date.issued | 2021-01-20 | |
| dc.description.abstract | Biopharmaceutical products are of great importance in the treatment or prevention of many diseases and represent a growing share of the global pharmaceutical market. The usual technology for protein synthesis (cell-based expression) faces certain obstacles, especially with ‘difficult-to-express’ proteins. Cell-free protein synthesis (CFPS) can overcome the main bottlenecks of cell-based expression. This review aims to present recent advances in the production process of biologic products by CFPS. First, key aspects of CFPS systems are summarized. A description of several biologic products that have been successfully produced using the CFPS system is provided. Finally, the CFPS system's ability to scale up and scale down, its main limitations and its application for biologics production are discussed. | en_US |
| dc.description.sponsorship | The authors thank the Coordination for the Improvement of Higher Education Personnel (CAPES, Brazil) for the MSc scholarships provided to CH Chiba (grant financial code 001). Prof. Adriano R, Azzoni and Prof. Marco Antonio Stephano were supported by Conselho Nacional de Desenvolvimento Cient´ıfico e Tecnologico – CNPq, Brazil (grants 304125 ´ /2018-0 and 313824/2019-3, respectively). This publication was financed by Fundac¸ ao de Aux ˜ ´ılio a Pesquisa do Estado de S ` ao Paulo (S ˜ ao Paulo Research Foundation – FAPESP), ˜ Brazil (grant 2018/15794-6). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. | en_US |
| dc.description.uri | https://www.future-science.com/doi/10.2144/btn-2020-0155 | en_US |
| dc.format.extent | 8 pages | en_US |
| dc.genre | journal articles | en_US |
| dc.identifier | doi:10.13016/m2q4ji-ttlz | |
| dc.identifier.citation | Chiba, Camila Hiromi; Knirsch, Marcos Camargo; Azzoni, Adriano Rodrigues; Moreira, Antonio R; Stephano, Marco Antonio; Cell-free protein synthesis: advances on production process for biopharmaceuticals and immunobiological products; BioTechniques 70,2 (2021); https://www.future-science.com/doi/10.2144/btn-2020-0155 | en_US |
| dc.identifier.uri | https://doi.org/10.2144/btn-2020-0155 | |
| dc.identifier.uri | http://hdl.handle.net/11603/21031 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Future Science Group | en_US |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Chemical, Biochemical & Environmental Engineering Department Collection | |
| dc.relation.ispartof | UMBC Office of the Provost | |
| dc.relation.ispartof | UMBC Faculty Collection | |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) | * |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
| dc.title | Cell-free protein synthesis: advances on production process for biopharmaceuticals and immunobiological products | en_US |
| dc.type | Text | en_US |