3D-Printed Microfluidic-Based Cell Culture System With Analysis to Investigate Macrophage Activation
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Date
2025-2-18
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Citation of Original Publication
Selemani, Major A., Giraso Keza Monia Kabandana, Chengpeng Chen, and R. Scott Martin. “3D-Printed Microfluidic-Based Cell Culture System With Analysis to Investigate Macrophage Activation.” ELECTROPHORESIS. February 18, 2025. https://doi.org/10.1002/elps.8109.
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This is the peer reviewed version of the following article: Selemani, Major A., Giraso Keza Monia Kabandana, Chengpeng Chen, and R. Scott Martin. “3D-Printed Microfluidic-Based Cell Culture System With Analysis to Investigate Macrophage Activation.” ELECTROPHORESIS. February 18, 2025. https://doi.org/10.1002/elps.8109., which has been published in final form at https://doi.org/10.1002/elps.8109. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
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Abstract
In this paper, we describe the development of 3D-printed microfluidic cell culture devices that can be coupled with a circulation system to study the dynamics of both intracellular and extracellular (release) processes. Key to this approach is the ability to quantitate key analytes on a minutes timescale with either on-line (in this study, quantitating nitric oxide production using an amperometric flow cell) or off-line (in this work, quantitating intracellular itaconate production with LC/MS) analytical measurements. To demonstrate the usefulness of this approach, we chose to study macrophage polarization as a function of the extracellular matrix (silk) fiber size, a major area of research in tissue engineering. It was found that the use of larger fibers (1280 nm vs. smaller 512 nm fibers) led to increases in the production of both nitric oxide and itaconate. These findings set the foundation for future research for the creation of finely tuned microfluidic 3D cell culture approaches in areas where flow and the extracellular matrix play a significant role in barrier transport and where integrated analysis of key markers is needed.