Measurement of Oxygen Concentrations in the Intact Beating Heart Using Electron Paramagnetic Resonance Spectroscopy: A Technique for Measuring Oxygen Concentrations in Situ

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dc.contributor.authorZweier, Jay L.
dc.contributor.authorThompson-Gorman, Susan
dc.contributor.authorKuppusamy, Periannan
dc.contributor.departmentBeverly K. Fine School of the Sciencesen_US
dc.date.accessioned2017-09-27T21:32:17Z
dc.date.available2017-09-27T21:32:17Z
dc.date.issued1991-12
dc.description.abstractElectron paramagnetic resonance (EPR) spectroscopy can be applied to measure oxygen concentrations in cells and tissues. Oxygen is paramagnetic, and thus it interacts with a free radical label resulting in a broadening of the observed linewidth. Recently we have developed instrumentation in order to enable the performance of EPR spectroscopy and EPR oximetry in the intact beating heart. This spectrometer consists of 1–2-GHz microwave bridge with the source locked to the resonant frequency of a specially designed lumped circuit resonator. This technique is applied to measure the kinetics of the uptake and clearance of different free radical labels. It is demonstrated that this technique can be used to noninvasively measure tissue oxygen concentration. In addition, rapid scan EPR measurements can be performed enabling gated millisecond measurements of oxygen concentrations to be performed over the cardiac cycle. Thus, low-frequency EPR spectroscopy offers great promise in the study of tissue oxygen concentrations and the role of oxygen in metabolic control.en_US
dc.description.urihttps://link.springer.com/article/10.1007/BF00786005en_US
dc.format.extent16 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/M2T14TQ28
dc.identifier.urihttps://doi.org/10.1007/BF00786005
dc.identifier.urihttp://hdl.handle.net/11603/5662
dc.language.isoen_USen_US
dc.publisherJournal of Bioenergetics and Biomembranesen_US
dc.titleMeasurement of Oxygen Concentrations in the Intact Beating Heart Using Electron Paramagnetic Resonance Spectroscopy: A Technique for Measuring Oxygen Concentrations in Situen_US
dc.typeTexten_US

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